拆方研究降香总萜、降香黄酮、丹参酮在冠心丹参方中抗大鼠心肌细胞缺氧/复氧损伤作用  

Separate Experimental Study of Effect of Lignum Acronylchiae Total Flavonoids,Lignum Acronylchiae Oil,Tanshinone in the Guanxin Danshen Capsule against Cardiomyocyts Anoxia/Reoxygenation Injury

作　　者：黄璜[1] 何欢[1] 王丹辉[2] 王金钱[2] 黄方威[2] 何秀菊[2] 刘丹[1] 范玫玫[2] 何明[1] 

机构地区：[1]南昌大学药学院药理学与分子治疗学教研室,南昌330006 [2]中药固体制剂制造技术国家工程研究中心,南昌330006

出　　处：《中国实验方剂学杂志》2012年第2期191-194,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部国际科技合作项目(2006DFB33080)

摘　　要：目的:研究冠心丹参处方中,分别除去降香总萜和降香总黄酮,或是加入丹参酮,研究其对抗心肌细胞缺氧/复氧(A/R)损伤的保护作用,探讨各组分对药效的贡献。方法:取培养4 d的大鼠乳鼠心肌细胞,随机分为:对照组(Cont组)、(1)A/R组、(2)1%的DSMO对照组、(3)冠心丹参组、(4)～(6)降香总萜与/和降香总黄酮阴性组、或是冠心丹参处方中分别加入终浓度为1,2,4 mg的丹参酮;检测细胞存活率、超氧化物歧化酶(SOD)、过氧化氢酶(catalase)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量及孵育液乳酸脱氢酶(LDH)活性。结果:在冠心丹参处方中分别除去降香总萜与/和降香总黄酮后,样品对抗心肌细胞的A/R的作用显著减弱,尤其以反映细胞抗脂质氧化状态指标SOD,catalase,GPx活性以及MDA含量更为明显;而加入丹参酮后,其对抗心肌细胞A/R的作用有不同程度的增强,尤其以反映细胞存活状态指标细胞存活率以及LDH更为明显,以2 mg丹参酮最为理想。结论:冠心丹参处方中在对抗心肌细胞A/R损伤的作用上,降香总萜和降香总黄酮不可缺如;加入2 mg丹参酮可增强其心肌保护作用。Objective： To investigate the protective effect on cardiomyocyts against anoxia/reoxygenation（A/R） injury in Guanxin Danshen compounding of the best ratio,if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed,or tanshinone from salvia miltiorrhiza was added,of the contribution of various components on the efficacy.Method： Taken cultured neonatal rat ventricular cardiomyocytes for 4 days were randomly divided into seven groups： Control group;（1） A/R group;（2） 1% DSMO group;（3） the group treated with the best ratio;（4）-（6）： these groups respectively treated with lignum acronylchiae oil and/or lignum negative total flavonoids,or salviol of different dose 1,2,4 mg added;cell viability,superoxide dismutase（SOD）,Catalase,glutathione peroxidase（GSH-Px） activities,malondialdehyde（MDA） contents and lactate dehydrogenase（LDH） activitied were detected.Result： In Guanxin Danshen compounding of the best ratio,the protective effect of cardiomyocyte against A/R injury was significantly decreaed if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed,these changes of anti-lipid oxidation indexes such as SOD,Catalase,GSH-Px activities and MDA contents were more obvious than other indexes;Once salviol was added,the protective effect on cardiomyocyte against A/R was enchanced with different degree,espcially LDH and cell viability was obvious and 2 mg salviol was the best protective effect.Conclusion： In the Guanxin Danshen compounding of the best ratio,lignum acronylchiae oil and lignum acronylchiae total flavonoids were necessary;Adding 2 mg salviol could enchance the protective effect on cardiomyocyte.

关 键 词：冠心丹参胶囊 心肌细胞 缺氧/复氧损伤 降香总萜 降香总黄酮 丹参酮 

分 类 号：R285.5[医药卫生—中药学]