银纳米有序阵列SERS活性基底的制备及在膀胱肿瘤细胞检测中的应用  

Fabrication of Silver Ordered Nanoarrays SERS-Active Substrates and Their Applications in Bladder Cancer Cells Detection

作　　者：刘悠[1] 黄丽清[1] 王军[1] 童慧敏[1] 袁林[1] 赵丽华[1] 张薇薇[1] 王磊[2] 朱键[3] 

机构地区：[1]西安交通大学理学院,陕西西安710049 [2]西安交通大学医学院第一附属医院,陕西西安710061 [3]西安交通大学生命科学与技术学院,陕西西安710049

出　　处：《光谱学与光谱分析》2012年第2期386-390,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(10804091);陕西省科学技术研究发展计划项目(2009K06-23)资助

摘　　要：以多孔阳极氧化铝(porous anodic alumina,PAA)膜为模板,采用真空电子束蒸镀技术,分别在PAA多孔层以及阻挡层表面形成了银纳米孔和银纳米帽有序阵列表面增强拉曼散射(surface-enhancedRaman scattering,SERS)活性基底,并以膀胱肿瘤细胞作为分子探针,测试和分析了这两种SERS活性基底的表面增强拉曼光谱的特性。结果表明,两种SERS活性基底对膀胱肿瘤细胞的拉曼散射信号均有很好的增强作用。银纳米帽有序阵列SERS活性基底不仅具有较高的SERS增强和荧光猝灭效应,而且不存在与PAA膜中草酸根杂质相关的干扰峰,可获得膀胱肿瘤细胞拉曼散射光谱的更多细节信息。Highly ordered silver nanopore and nanocap arrays,which were used as surface-enhanced Raman scattering active（SERS-active） substrates,were fabricated by electron-beam evaporating silver on the surface of porous layer and barrier layer of porous anodic alumina（PAA） membranes,respectively.The SERS characteristics of the SERS-active substrates were tested with bladder cancer cells as molecular probe.The results indicated that both the SERS-active substrates displayed a strong SERS enhancement effect.The silver nanocap ordered arrays SERS-active substrate displayed not only higher SERS and fluorescence quenching effect,but also no interferential spectrum related with oxalate impurity remaining in PAA membranes,and therefore can result in the high quality Raman spectroscopy of bladder cancer cells.

关 键 词：多孔阳极氧化铝膜 银有序纳米结构阵列 表面增强拉曼散射 膀胱肿瘤细胞 

分 类 号：O657.3[理学—分析化学]