HPLC-ESI/MS分析5-羟基黄酮在大鼠体内的代谢产物  

Metabolites Analysis of 5-Hydroxyflavone in Rat by HPLC-ESI/MS

作　　者：韦英杰[1] 姜金生[2] 谭晓斌[1] 陈斌[1] 刘炜[1] 胡春萍[1] 马世平[2] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,南京210028 [2]中国药科大学中药药理教研室,南京210009

出　　处：《中国现代应用药学》2012年第1期60-63,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金资助项目(30973978)

摘　　要：目的研究5-羟基黄酮在大鼠体内的代谢产物。方法应用高效液相色谱-电喷雾质谱(HPLC-ESI/MS)检测大鼠灌胃5-羟基黄酮后血浆、尿液、胆汁和粪便中的代谢产物。实验采用Zorbax C18色谱柱,0.05%甲酸乙腈-0.05%甲酸水二元线性梯度洗脱进行色谱分离,并与电喷雾质谱联用,根据正离子模式的分子离子峰获得化合物分子量信息,推测化合物的可能结构。结果仅在大鼠粪便中检测到原形成分,在大鼠尿液、粪便、血浆、胆汁中检测到5-羟基黄酮葡糖醛酸结合物。结论 5-羟基黄酮吸收差,在大鼠体内主要以II相代谢产物葡萄糖醛酸结合物的形式存在。OBJECTIVE To study the metabolites of 5-hydroxyflavone in rat. METHODS High performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI-MS） was used for analysis of metabolites of 5-hydroxyflavone in rat after oral administration. The separation was performed with a Zorbax C18 column using a binary gradient elution of 0.05% formic acid acetonitrile-0.05% formic acid water. The quasi-molecular ions of compounds in positive mode were observed for molecule mass information, and the potential structures were presumed. RESULTS Parent component 5-hydroxyflavone was found only in rat fence, while glucuronide conjugates of 5-hydroxyflavone were found in rat urine, fence, plasma and bile. CONCLUSION Absorption of 5-hydroxyflavone is poor, and the major forms of 5-hydroxyflavone in rat are metabolites of phase II glucuronide conjugates.

关 键 词：5-羟基黄酮 代谢 大鼠 高效液相色谱-电喷雾质谱 

分 类 号：R917.1[医药卫生—药物分析学]