大口黑鲈北方亚种和佛罗里达亚种及其杂交子代的遗传分析  

Genetic analysis of northern largemouth bass,Florida largemouth bass,and their reciprocal hybrids

作　　者：蔡磊[1,2] 白俊杰[1,2] 李胜杰[1] 陈昆慈[1] 樊佳佳[1] 马冬梅[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2012年第1期70-76,共7页Journal of Fishery Sciences of China

基　　金：农业部948项目(2010Z9),农业部公益性行业科研专项(200903045);国家科技支撑计划项目(2006BAD01A1209);广东省科技计划项目(2007B020708008)

摘　　要：用18对微卫星引物对大口黑鲈北方亚种（Micropterus salmoides salmoides,N）、佛罗里达亚种（M.salmoides floridanus,F）及其正交子代（N♀×F♂）和反交子代（F♀×N♂）进行遗传多样性和遗传结构分析。结果表明,18对引物扩增出的等位基因数为2~8个,平均等位基因数为5.0。检测到6对（Jzl48、Jzl68、Jzl84、MiSaTPW76、Msal21、Mdo6和Mdo7）亚种间特异性引物,其中有2对引物（Jzl48和Mdo7）可以用来鉴别大口黑鲈北方亚种、佛罗里达亚种和其杂交子代。平均有效等位基因数、平均期望杂合度和平均多态信息含量均为杂交组合F♀×N♂最高,分别为3.199 7,0.638 9和0.570 6,平均观测杂合度为杂交组合N♀×F♂最高（0.848 8）。对亲代与杂交子代间的遗传分化分析表明,正反交子代均与北方亚种的遗传分化最小（0.092和0.119 6）。基于Nei’s遗传距离构建的UPGMA系统进化树显示正交子代N♀×F♂与母本N聚为一支,反交子代F♀×N♂与父本N聚为一支。We evaluated the genetic variation and genetic structure of northern largemouth bass,Micropterus sal-moides salmoides（N）,Florida largemouth bass,M.S.floridanus（F）,and their obverse（N♀×F♂） and inverse（F♀×N♂） crosses,using 18 pairs of microsatellite primers.We generated between 2–8 alleles at each locus,with a mean allele number of 5.0.Six subspecies specific loci（Jzl48,Jzl68,Jzl84,MiSaTPW76,Msal21,Mdo6 and Mdo7） could be used to identify the two subspecies.Moreover,2 loci（Jzl48,Mdo7） could be used to distinguish parents and their reciprocal hybrids.The number of effect alleles（Ne）,expected heterozygosity（He）,and polymorphic information content were all highest in F♀×N♂（3.199 7,0.638 9,0.570 6,respectively）.The observed heterozy-gosity（Ho） was highest in N♀×F♂（0.848 8）.FST analysis of all groups and loci revealed that both reciprocal hy-brids had lower genetic differentiation with N species.The UPGMA clustering analysis suggested that the off-spring was nearest to the female brood stock for the obverse cross and nearest to the male brood stock for the in-verse cross,suggesting that northern largemouth bass pass more genetic material than Florida largemouth bass.

关 键 词：大口黑鲈北方亚种 佛罗里达亚种 杂交 微卫星标记 遗传关系 

分 类 号：S917[农业科学—水产科学]