枸杞多糖对热应激大鼠生精细胞凋亡的保护作用及其机制研究  

Protective effect of lycium barbarum polysaccharides against heat stress-induced germ cell apoptosis in rats and its mechanism

作　　者：谭秋慧[1] 安长新[1] 萧云[2] 廖志敏[2] 

机构地区：[1]广东医学院组织学与胚胎学教研室,广东湛江524023 [2]广东医学院附属医院神经内科,广东湛江524023

出　　处：《中华男科学杂志》2012年第1期88-92,共5页National Journal of Andrology

摘　　要：目的:研究枸杞多糖对热应激所致大鼠生精细胞凋亡的保护作用及其作用机制。方法:雄性SD大鼠90只,随机分为正常对照组、热应激组(HS)、枸杞多糖高(HLBP)、中(MLBP)、低(LLBP)剂量组[剂量分别为100、50、10 mg/(kg.d)],每组18只。按以上剂量给枸杞多糖组大鼠连续灌胃14 d,正常对照组和热应激组给予等量生理盐水灌胃。第15 d给予热应激组和各枸杞多糖组大鼠43℃水浴30 min。热应激后24 h、48 h和7 d颈椎脱臼处死大鼠,TUNEL法检测生精细胞凋亡,免疫组化法检测Caspase-3蛋白的表达,ELISA法检测睾丸组织胞质细胞色素C(cytochrome C,Cyt C)浓度。结果:与热应激组比较,各枸杞多糖组的凋亡指数均明显降低(P<0.05),免疫组化显示生精细胞Caspase-3阳性表达明显减少(P<0.05),胞质Cyt C浓度显著降低(P<0.05)。结论:枸杞多糖能抑制Cyt C自线粒体释放出来,使Caspase-3的活化减少,导致生精细胞的凋亡减少,推断其通过线粒体途径抑制生精细胞的凋亡而起到保护睾丸组织的作用。Objective： To investigate the protective effect of lycium barbarum polysaccharides（LBP） against heat stress-induced apoptosis of germ cells in rats and its action mechanism.Methods： Ninety male Sprague-Dawley rats were randomly divided into five groups of 18 each： control,heat stress（HS）,high-dose LBP,median-dose LBP and low-dose LBP.The rats of the three LBP groups were given LBP by intragastric administration at 100 mg/（kg·d）,50 mg/（kg·d） and 10 mg/（kg·d） respectively for 14 days,and on the 15th day they,together with those of the HS group,were exposed to a heat of 43 ℃ for 30 minutes.At 24 h,48 h and 7 d after heat stress,the animals were killed by cervical dislocation,followed by observation of the apoptotic germ cells by TUNEL,determination of the expression of Caspase-3 by immunohistochemistry and detection of cytochrome C in the cytosol by ELISA.Results： Compared with the HS group,the three LBP groups showed statistically significant decreases in the apoptosis index（P〈0.05）,the expression level of Caspase-3 in germ cells（P〈0.05） and the concentration of cytochrome C in the cytosol（P〈0.05）.Conclusion： LBP can inhibit cytochrome C release from mitochondria,decrease the expression of Caspase-3 and hence reduce the apoptosis of germ cells.It thus can be deduced that LBP can protect germ cells against apoptosis via the mitochondrial pathway.

关 键 词：枸杞多糖 热应激 生精细胞 凋亡 细胞色素C 

分 类 号：R285.5[医药卫生—中药学]