高原肺水肿的血浆蛋白质组学研究  

Study on plasma proteomics in patients with high altitude pulmonary edema(HAPE)

作　　者：罗勇军[1] 陈郁[2] 高钰琪[2] 

机构地区：[1]第三军医大学高原军事医学系高原疾病学教研室、高原医学教育部重点实验室,重庆400038 [2]第三军医大学病理生理学与高原生理学教研室

出　　处：《解放军医学杂志》2012年第1期31-33,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(30900715);国家科技支撑计划(2009BAI85B01);国家973项目(2012CB518200)~~

摘　　要：目的探讨高原肺水肿患者血浆蛋白组中差异表达的蛋白质及其意义。方法抽取拉萨市(海拔3658m)6例高原肺水肿患者和6例健康对照者的血浆,去除血浆高丰度蛋白,采用2-DE分离血浆/血清低丰度蛋白质,酶解差异表达的蛋白质斑点,采用MALDI-TOF/MS检测肽指纹谱,并在GenBank数据库中进行比对分析。结果与对照组比较,高原肺水肿患者免疫球蛋白κ1轻链、血清转铁蛋白前体、α-胰蛋白酶抑制剂重链相关蛋白表达上调,人纤维胶凝蛋白3表达下调。结论高原肺水肿患者血浆中上述4种蛋白的差异表达可能与高原肺水肿的发生有关,有可能成为预测该疾病的新靶点。Objective To investigate the differential expressions of protein in the plasma proteome in patients suffering from high altitude pulmonary edema （HAPE） and their implications. Methods The plasma of six HAPE patients and six healthy controls was studied. The high-abundant proteins in plasma were removed. The low-abundant proteins in the plasma/serum were segregated by 2-DE. MALDI-TOF/MS was adopted to measure the peptide dactylogram after the differential proteins spots were digested by enzymes. Comparison and analysis were made in the GeneBank. Results The immunoglobulin K1 light chain, serum transferrin protein precursorj and oe -trypsin inhibitor heavy chain-related protein expression were increased in HAPE patients compared with those of the control group. However the human fibrin glue coagulation protein 3 was down-regulated. Conclusion The differential expression of the above four proteins in the plasma of HAPE patients may be related to the occurrence of HAPE, and can be used as the target spots for the prediction of HAPE.

关 键 词：高原肺水肿 蛋白质组学 生物标记 血浆 

分 类 号：R56[医药卫生—呼吸系统]