5-HT_7受体激动剂改善脊髓损伤大鼠排尿障碍  

Improving the voding disfunction by a 5-HT_7 receptor agonist in rats with chronic spinal cord injury

作　　者：吴刚[1] 曹海兵[2] 程曙杰[3] 陈加生[1] 谷宝军[1] 

机构地区：[1]上海交通大学医学院附属第六人民医院泌尿科,上海200233 [2]苏州大学 [3]上海市长宁区中心医院

出　　处：《临床泌尿外科杂志》2012年第1期68-71,共4页Journal of Clinical Urology

基　　金：国家自然科学基金资助项目(No:30872568)

摘　　要：目的:研究五羟色胺-7受体激动剂对慢性脊髓损伤(spinal cord injury,SCI)大鼠排尿障碍的改善作用。方法:体重175～200g之间的SD雌性大鼠,脊髓损伤模型7只,正常对照8只。乌拉坦麻醉下,在正常大鼠和脊髓损伤的模型的大鼠颈静脉和膀胱内置管,连接压力感受器,记录膀胱最大容量、剩余尿量、排尿量和尿道外括约肌的肌电活动。静脉注入5-HT7受体激动剂LP44(3～300μg/kg)得到剂量-效应曲线随后再给予5-HT7受体抑制剂SB269970(100μg/kg)。结果:LP44剂量依赖性的降低SCI大鼠膀胱最大容量,并且增加排尿量、减少剩余尿量从而增加排尿效率。但是不能引起脊髓完好的大鼠排尿的改变。SB269970注入后可以逆转LP44的作用。同时LP44引起脊髓损伤大鼠尿道外括约肌强直收缩中出现阶段性的松弛,而对于脊髓完好的大鼠作用无明显改变。结论:LP44可以剂量依赖性地部分恢复SCI大鼠的尿道外括约肌协调性松弛,从而降低膀胱容量,增加排尿量,减少剩余尿量,结果增加排尿效率,改善排尿障碍。Objective：To investigate the effect of 5-hydroxytryptamine serotonin 7 receptor agonists on mieturi- tion dysfunction in rats with chronic spinal cord injury（SCI）. Method： Intact rats and SCI rats were catheterized through the bladder dome for saline-filling cystometry. Dose-response curves for i. v. LP44 were followed each by SB269970. The capacity,residual volume, micturition volume,and voiding pressure were measured. Result： Intact rats showed few significant changes in eystometrie variable. SCI rats responded to LP44 with dose-dependent inn crease in micturition volume and decrease in capacity, residual volume and voiding pressure, resulting in improve- ment in voiding efficiency. Effects of LP44 were reversed by SB269970. Conclusion： LP44 is of some help to im- provement in voiding efficiency through induced emergence of phasic EUS relaxation.

关 键 词：脊髓损伤 大鼠 排尿 5-羟色胺受体 

分 类 号：R651.2[医药卫生—外科学]