靶向包载经PEG修饰的重组腺病毒PLGA造影剂的制备及其体外寻靶实验  

Preparation of Targeted Pegylated Adenoviral Vectors From Plga-cooh and Targeting Study in Vitro

作　　者：叶鸣[1] 王志刚[1] 李兴升[2] 冉海涛[1] 骆杰[1] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆市400010 [2]重庆医科大学附二院老年科

出　　处：《中国超声医学杂志》2012年第1期1-4,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金青年科学基金(No.30800271);国家自然科学基金面上项目(No.30770565)

摘　　要：目的采用共价结合的方法制备靶向包载经PEG修饰的重组腺病毒PLGA-COOH造影剂,并考察其体外寻靶能力。方法采用碳二亚胺法将包载经PEG修饰的重组腺病毒PLGA-COOH造影剂与抗体共价结合,制备靶向高分子超声造影剂,检测该造影剂一般特性及体外寻靶能力。结果体外寻靶实验显示,该靶向超声造影剂与普通包载经PEG修饰的重组腺病毒PLGA COOH造影剂对照能聚集并牢固结合到人脐静脉血管内皮细胞表面。结论成功制备出靶向包载经PEG修饰的重组腺病毒PLGA-COOH造影剂,该靶向造影剂在体外具有较强的特异性寻靶结合能力。Objective To prepare a kind of targeted PEG-ADV-loaded PLGA-COOH covalently binding with monoclonal antibody and investigate its affinity for Human umbilical vein endothelial cell （HUVEC） in vitro. Methods Targeted PLGA-COOH were prepared by combining PLGA-COOH with anti-Eselectin monoclonal antibody using 1-ethyl-3-（3-dimethylaminopropyl） carbodi-imide hydrochloride （EDC） and N-hydroxysuccinimide （NHS） mediated cross linking agents. The characteristic of targeting to HUVEC and served non-targeted PLGA- COOH as the control group were valued. Results In the targeting study in vitro, it was shown that many targeted PLGA COOH ultrasound contrast agents conjugated with HUVEC tightly. Conclusions Targeted PEG-ADV-loaded PLGA-COOH were prepared successfully and can bind to HUVEC effectively in vitro.

关 键 词：共价结合 PLGA—COOH造影剂 靶向 

分 类 号：TQ421.7[化学工程]