高强度聚焦超声治疗后荷瘤鼠脾淋巴细胞的免疫治疗作用  

Immune Therapeutic Effects of Lymphocytes After High Intensity Focused Ultrasound Treatment of Mice Bearing H_(22) Hepatic Carcinoma

作　　者：夏纪筑[1] 冉立峰[1] 谢方林[1] 伍烽[1] 

机构地区：[1]重庆医科大学生物医学工程系、重庆市生物医学工程学重点实验室、重庆市超声医学工程重点实验室省部共建国家重点实验室培育基地,重庆市400016

出　　处：《中国超声医学杂志》2012年第1期13-16,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家教育部创新团队发展计划(No.IRT0454);国家自然科学基金重点项目(No.30830040)

摘　　要：目的探讨高强度聚焦超声(high intensity focused ultrasound,HIFU)治疗H_(22)移植性肝癌后活化的淋巴细胞对早期同种肿瘤的治疗作用及临床意义。方法 32只C57BL/6J近交系H_(22)荷瘤鼠随机分为HIFU组和HIFU假照组,每组16只。分别在H_(22)肝癌移植后7 d接受HIFU治疗,HIFU假照和观察(对照组)。HIFU治疗后14 d,应用乳酸脱氢酶(1actate dehydrogenase,LDH)释放法测定各组鼠在不同效靶比脾淋巴细胞对H_(22)肿瘤细胞体外杀伤活性;90只C57BL/6J H_(22)荷瘤鼠随机分为HIFU组、HIFU假照组和对照组,分别接受3组提取的淋巴细胞静脉注射。观察各组小鼠肿瘤消退率、肿瘤体积、肿瘤转移发生率及生存率。结果与假照组和对照组比较,HIFU组对H_(22)细胞杀伤活性明显增强,差异有统计学意义(P<0.01)。接种第60天,与假照组和对照组比较,HIFU组肿瘤消退率明显增加(P<0.01);HIFU组肿瘤体积增长缓慢,而假照组与对照组基本相同;与HIFU组比较,假照组和对照组转移率随着病程进展明显增加,差异有统计学意义(P<0.01);与假照组和对照组比较,HIFU组存活率明显增加(P<0.01)。结论 HIFU治疗H_(22)移植性肝癌后,活化的淋巴细胞对早期同种移植性肿瘤有治疗作用。Objective To investigate the immune therapeutic effects of HIFU-activated T lymphocytes after adoptive immunotherapy in the homogeneous tumor-bearing mice. Methods Thirty-two C57BL/6J normal mice received HIFU, sham-HIFU exposure on 7 day after tumor implantation. Fourteen days after HIFU treatment, spleen lymphocytes were extracted in each treated-mouse. In addition, spleen lymphocytes extracted from 16 C57BL/6J normal mice were used as a control. Using LDH assay, the cytotoxic activity of the lymphocytes against H22 tumor cells was assessed in vitro in each group. Ninety C57BL/6J mice bearing H22 tumor were randomly divided into HIFU group, sham HIFU group and control group. They underwent intravenous injection via the tail vein respectively. The tumor extinction rates, tumor volume, tumor metastatic rates and tumor survival rates were observed in 60 days. Results Compared to the values in both sham-HIFU and control groups, there were significant increase of the cytotoxicity against H22 tumor cells in HIFU group （P〈0.01）. Sixty-day tumor extinction rates of mice were 56% in HIFU group, 25% in sham-HIFU and 18~//00 in control. Tumor volume were significantly smaller in HIFU group than the other groups. Tumor metastatic rates were significantly decresed in HIFU group than the other groups. Tumor sur vival rates were 75% in HIFU group, 34% in sham-HIFU and 17% in control. Conclusions There is markedly immune therapeutic effects of HIFU-activated CTLs on homogeneous tumor-bearing mice in early stage.

关 键 词：高强度聚焦超声 肿瘤 治疗 淋巴细胞 

分 类 号：R445.1[医药卫生—影像医学与核医学] R730.51[医药卫生—诊断学]