杂种卡特兰花粉萌发和花粉贮藏性研究  

Germination and Preservation of Rhyncholaeliocattleya Pollen

作　　者：郑宝强[1] 王雁[1] 彭镇华[1] 李莉[2] 

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [2]河北农业大学园艺学院,河北保定071001

出　　处：《热带亚热带植物学报》2012年第1期13-18,共6页Journal of Tropical and Subtropical Botany

基　　金：中央级公益性科研院所专项基金(2724-14);北京市园林绿化局计划项目(Y1HH200900304)资助

摘　　要：以杂种卡特兰品种‘绿世界’(Rhyncholaeliocattleya Sung Ya Green‘Green World’)的新鲜花粉为试材,采用正交试验比较了蔗糖、H3BO3、CaCl2对花粉萌发的影响。结果表明,适宜的花粉培养基为:蔗糖100 gL-1+H3BO3 40 mgL-1+CaCl2 150 mgL-1。不同贮藏方法对杂种卡特兰花粉萌发率的影响较大,4℃低温湿润贮藏30 d后,花粉萌发率仅为3.5%,而4℃低温干燥贮藏、-20℃冷冻湿润和干燥贮藏720 d后,花粉仍然具有较高的活力,花粉萌发率均达32.5%以上,且对授粉结实率没有明显影响。杂种卡特兰的授粉最佳时期为开花后0-8 d,花粉块在整个花期均保持较高的活性。The effects of sucrose, boron and calcium on the germination of Rhyncholaeliocattleya Sung Ya Green ＇ Green World＇ pollen were studied by orthogonal design experiment. The results showed that the optimum culture medium for pollen germination was 100 g L^-1 sucrose ＋40 mg L^-1 H3BO3 ＋ 150 mg L^-1 CaCl2. Different storage methods had different effects on pollen viability of ‘Green World＇. The pollen germination rate was only 3.5% humid stored under 4℃ for 30 days. However, after dry stored in 4℃, freeze-wetting and freeze-drying stored in -20℃ for 720 days, respectively, the pollen germination rate still maintained at high level, reaching up to above 32.5%, and there was not effect on seed setting. The best time for pollination was 0-8 days after flowering and the pollinium had high vitality during whole florescence.

关 键 词：杂种卡特兰 花粉活力 花粉贮藏 可授性 

分 类 号：S682.31[农业科学—观赏园艺]