猪源性乙型脑炎病毒分离株全基因序列测定和分析  

Complete sequence analysis of Japanese encephalitis virus JX61 strain from swine

作　　者：赵灵燕[1] 谢荣辉[2] 朱函坪[2] 徐芳[2] 杨章女[2] 姚苹苹[2] 周小龙[2] 朱智勇[2] 

机构地区：[1]浙江省动物疫病预防控制中心,杭州310020 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国人兽共患病学报》2012年第1期37-40,共4页Chinese Journal of Zoonoses

基　　金：浙江省医药卫生科学研究基金项目(2008B39)

摘　　要：目的了解猪源性乙型脑炎病毒(乙脑病毒)的基因特征。方法对猪源性乙脑病毒株(JX61)进行全基因组序列测定和基因进化特性的分析。结果猪源性乙脑病毒JX61株全基因组全长均为10 964个核苷酸,含有一个开放阅读框架,编码3 432个氨基酸。与GenBank中选择的41株乙脑病毒全基因序列比较发现,其核苷酸同源为83.6%～99.3%,氨基酸总体同源性为94.9%～99.6%。通过全基因序列进行系统进化分析均显示该毒株属于基因Ⅰ型乙脑病毒。结论猪源性乙脑病毒JX61株属于基因I型,与国内分离株XJP613关系最为接近。提示猪体内的乙脑病毒基因型别有了新的变化,这也是浙江省在猪体第一次分离基因Ⅰ型乙脑病毒。In order to understand the genetic characteristics of Japanese encephalitis virus from swine,an JEV was isolated from swine,and its complete genome was sequenced and analyzed comparatively.The results of sequencing of the nucleotides demonstrated that the genome of strain JX61 was 10964 nucleotides in length with a single open reading frame encoding 3432 amino acids.Comparison of the complete genome sequences of different JEV isolates showed JX61 was 83.6%-99.3% nucleotide sequence homology among them,which resulted in 94.9%-99.6% amino acid sequence homology.Phylogenetic analysis through full-length genome showed that the JX61 strain belonged to genotype Ⅰ.The phylogenetic analysis based on the entire genome demonstrated that JX61 strain has the nearest genetic relationship with XJP613 and the JX61 might belong to the genotype I.It was the first isolation of Japanese encephalitis virus genotype I from swine in Zhejiang province during recent years.

关 键 词：乙脑病毒 序列分析 种系发生 

分 类 号：R373.3[医药卫生—病原生物学]