血管活性肠多肽调控大鼠阴茎勃起的年龄及雄激素相关性研究  

The effect of aging and androgen on penile erection regulated by vasoactive intestinal peptide

作　　者：张存明[1] 沈周俊[1] 张敏光[1] 钟山[1] 王先进[1] 胡明明[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,上海200025

出　　处：《现代泌尿外科杂志》2012年第1期10-13,共4页Journal of Modern Urology

基　　金：国家自然科学基金面上项目基金资助(No:30872576)

摘　　要：目的建立不同月龄及不同雄激素水平的SD大鼠模型,评价血管活性肠多肽(VIP)调控不同模型大鼠阴茎勃起的作用。方法 SD雄性大鼠80只随机等分为4组:A组(对照组);B组(去势组),行双侧睾丸切除;C组(老龄组),普通饲养16个月;D组(去势老龄组),去势术后饲养16个月。A和B组1月后测勃起功能;C和D组16个月后测勃起功能。电刺激海绵体神经时海绵体内压力(ICP)的最大值与对应平均动脉压(MAP)的比值表示勃起功能,记为Ratio。海绵体注射VIP前、后大鼠勃起功能分别以b-Ratio和m-Ratio表示。m-Ratio与b-Ratio的比值(记为mR/bR)表示VIP对勃起功能的影响程度。留取血清,ELISA方法检测血清睾酮浓度。结果 A、B、C和D组的b-Ratio(%)分别为69.8±7.0、36.8±5.4、53.2±8.5、33.2±6.3;m-Ratio(%)分别为73.0±8.7、63.4±9.6、79.7±10.8、67.0±11.8;mR/bR分别为1.05±0.03、1.72±0.08、1.56±0.29、2.06±0.44。后3组与A组比较勃起功能增加程度明显(P均<0.01)。A、B、C和D组的血清睾酮浓度(ng/mL)分别为1.542±0.131、0.438±0.096、0.840±0.153、0.416±0.101,后3组均明显低于A组(P均<0.01)。结论年龄增长和雄激素水平降低导致大鼠勃起功能下降,年龄对勃起功能的影响可能基于雄激素水平的变化;VIP对阴茎勃起的调控作用与年龄增长呈正相关,与雄激素水平负相关。Objective To establish the rat model with different ages and androgen levels and to evaluate the effect of vasoactive intestinal peptide（VIP） on erectile function.Methods Eighty male SD rats were randomly divided into 4 groups with 20 in each,including group A（control）,group B（castration）,group C（aging） and group D（aging with castration）.And then,according to grouping,they were subjected to intracavernous injection of VIP at different times respectively.Erectile function was tested by recording intracavernosal pressure（ICP） and mean arterial blood pressure（MAP） directly before and after injection,expressed by ICP/MAP×100%（Ratio）.The ratio of ICP/MAP before and after injection was recorded as b-Ratio and m-Ratio,and improvement of erectile function was represented by m-Ratio/b-Ratio（mR/bR）.In the end of the experiment,blood samples were obtained by cardiac puncture and the level of serum testosterone were determined by ELISA.Results The b-Ratio（%） of group A,B,C and D was 69.8±7.0,36.8±5.4,53.2±8.5 and 33.2±6.3;m-Ratio（%） was 73.0±8.7,63.4±9.6,79.7±10.8 and 67.0±11.8;mR/bR was 1.05±0.03,1.72±0.08,1.56±0.29 and 2.06±0.44,respectively.The mR/bR of group B,C,and D were significantly higher than of group A（P0.01）.The serum testosterone concentrations（ng/mL） of group A,B,C and D was 1.542±0.131,0.438±0.096,0.840±0.153 and 0.416±0.101,and the value of group B,C,D was significantly lower than that of group A（P0.01）.Conclusions In rat models,aging and decline in androgen levels can cause erectile dysfunction and the effect of aging on erectile function may be based on the changes of androgen levels.The role of VIP,which can enhance erectile function,is positively correlated with aging and negatively regulated by androgen.

关 键 词：血管活性肠多肽 阴茎勃起 老龄 雄激素 勃起神经递质 

分 类 号：R698.1[医药卫生—泌尿科学]