咖啡酸对同型半胱氨酸诱导的白细胞炎症介质分泌的影响  

Effect of Caffeic Acid on Secretion of Inflammatory Mediators in Leukocytes Induced by Homocysteine

作　　者：赵海苹[1,2] 王宪[2] 冯娟[2] 卫晓红[1,3] 孙凯[1,3] 韩晶岩[1,4,3] 

机构地区：[1]北京大学医学部天士力微循环研究中心,北京100191 [2]北京大学基础医学院生理与病理生理学系,北京100191 [3]国家中医药管理局微循环三级实验室,北京100191 [4]北京大学基础医学院中西医结合研究室,北京100191

出　　处：《中药新药与临床药理》2012年第1期38-41,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重大新药创制科技重大专项(2008ZX09401-006);国家重点基础研究(973)项目(2011CB503904)

摘　　要：目的研究咖啡酸(caffeic acid,CA)对同型半胱氨酸(homocysteine,Hcy)诱导的小鼠外周血白细胞炎症介质分泌的影响。方法分离8周龄雄性C576J/BL小鼠外周血白细胞,用Hcy(200μmol/L)和CA(100μmol/L)处理30 min。用流式微球法检测白细胞培养基中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)的水平。用激光共聚焦显微镜观察白细胞中髓过氧化物酶(myeloperoxidase,MPO)的表达。用western blot检测白细胞中磷酸化的细胞外信号调节激酶(phospho-extracellu-lar signal-regulated protein kinase 1/2,p-ERK1/2)蛋白的表达。结果小鼠外周血白细胞用Hcy处理后,培养基中TNF-α水平没有显著的变化,而MCP-1水平显著升高,白细胞MPO分泌增加,p-ERK1/2蛋白表达显著增加。CA抑制了Hcy诱导的小鼠外周血白细胞炎症介质MCP-1和MPO的分泌,以及p-ERK1/2蛋白表达的增加。结论CA可以抑制Hcy诱导的小鼠外周血白细胞MCP-1和MPO的释放,该作用可能与其抑制p-ERK1/2的表达有关。Objective To investigate the influence of caffeic acid（CA） on the secretion of inflammatory mediators in mice leukocytes induced by homocysteine（Hcy）.Methods The peripheral blood leukocytes were isolated from Male C576J/BL mice of 8 weeks old,and then were treated with Hcy（200 μmol/L） and CA（100μmol/L） for 30 min.The levels of tumor necrosis factor-α（TNF-α） and monocyte chemoattractant protein-1（MCP-1） in the medium of leukocytes were detected by flow cytometric bead array.The expression of myeloperoxidase（MPO） was observed by confocal microscopy,and the expression of phosphorylated extracellular signal-regulated protein kinase 1/2（p-ERK） was detected by western blot.Results After treatment with Hcy,TNF-α in the medium remained unchanged,but the secretion of MCP-1 and MPO in leukocytes was increased.CA reduced Hcy-induced secretion of MCP-1 and MPO from peripheral blood leukocyte of mice,and depressed the expression of p-ERK1/2 in leukocytes.Conclusion CA depresses Hcy-induced secretion of inflammatory mediators from leukocytes,which is probably related with the inhibition of p-ERK1/2 expression.

关 键 词：咖啡酸 同型半胱氨酸 白细胞 炎症因子 磷酸化细胞外信号调节激酶 

分 类 号：R285.5[医药卫生—中药学]