黑龙江省西部地区玉米大斑病菌生理小种鉴定及生物学特性分析  

Identification of Helminthosporium turcicum Race in Western of Heilongjiang Province and Their Biological Characteristics Analysis

作　　者：浦子钢[1] 

机构地区：[1]黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔161005

出　　处：《黑龙江农业科学》2012年第1期45-50,共6页Heilongjiang Agricultural Sciences

基　　金：齐齐哈尔市政府科学技术研究基金资助项目(NYGG-0805)

摘　　要：为筛选和创制玉米抗病种质资源提供试验依据,研究鉴定了黑龙江省西部地区玉米大斑病生理小种类别,并对其生物学特性进行系统性的研究。结果表明:在黑龙江省西部地区发生的玉米大斑病生理小种为Ht3/Ht1、Ht2、HtN型生理小种,得出此病原菌菌丝最适合生长的pH为7,适合生长温度为20～30℃,菌落在查氏(NaNO3)培养基上生长优于查氏〔(NH4)2SO4〕培养基,菌丝生长速率在玉米面培养基中最大,平均生长率为1.55cm.d-1,蔗糖为碳源时生长最快,生长速率为1.73cm.d-1,以NaNO3为氮源时菌落生长最快,生长速率为1.70cm.d-1。在影响孢子萌发的因素中,pH为7时,孢子萌发率达到最高;在23～35℃时,不同碳源和氮源对孢子萌发影响不大,孢子萌发率均可达70%以上。In order to provide basis for selecting and creating maize germplasm resource with disease resistance, the physiological race categories of Helminthosporium turcicum in western of Heilongjiang province were identified and their biological characteristics were systematically studied. The results showed that the physiological races were Ht3/Ht1. Ht2 and HtN, the optimal pH for growth was 7, suitable temperature for growth was 20-30℃. Bacterial colony in Czapek（NaNO3 ）was better than in Cha[（NH4 ）2 SO4 ）medium, mycelial growth rate in the cornmeal medium was the largest,the average growth rate was 1.55 cm＇d-1 , the fastest growth was in the treatment taken sucrose as carbon source, the growth rate was 1.73 cm· d-1 , the fastest growth of colonies was in the treatment of taken NaNOa as nitrogen source,the growth rate of 1.70 cm.d-1. In the factors affecting the germination of spores,the highest germination rate was appeared when pH was 7. Different carbon and nitrogen sources had little effect on spore germination in 23-35℃, spore germination rate could reach above 70 %.

关 键 词：玉米大斑病 生理小种 生物学特性 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]