电针肝俞、期门对肝气郁结模型大鼠行为学的影响  

Effect of Electroacupuncture of Acupoint Ganshu and Qimen on Behavior Changes in Rats with Stagnation of Liver-qi

作　　者：赵海滨[1] 刘子旺[1] 张秀静[1] 

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《广州中医药大学学报》2012年第1期30-33,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81072772);首都医学发展科研基金资助项目(编号:SF-2007-Ⅲ-06)

摘　　要：【目的】探讨电针肝俞、期门穴对肝气郁结模型大鼠行为学的影响。【方法】将30只Wistar大鼠随机分为正常组、模型组、电针组(电针肝俞、期门穴),采用慢性不可预知温和应激结合孤养方法复制肝气郁结证大鼠模型,分别于实验前后进行开野实验(Open-Field Test)、蔗糖水偏嗜实验,同时于实验前1 d及实验第4、8、12、16、20、24、28天测量各组大鼠体质量及24 h摄食量。【结果】实验14 d后,模型组大鼠体质量增加缓慢,摄食量下降,与正常组比较差异有统计学意义(P<0.05或P<0.01),实验20 d后,电针组大鼠体质量、摄食量显著高于模型组(P<0.05)。在实验28 d发现,与正常组比较,模型组Open-Field Test水平运动得分和垂直运动得分、蔗糖水绝对消耗及偏嗜度均显著减少,电针组上述指标水平升高,与模型组比较差异均有统计学意义(P<0.05或P<0.01)。【结论】电针肝俞、期门穴对肝气郁结大鼠行为学改变具有一定防治作用。Objective To investigate the effect of electroacupuncture（EA） on behavior changes in rats with liver-qi stagnation syndrome.Methods Thirty Wistar rats were equally randomized into normal group,model group,and EA group（EA on acupoints of Ganshu and Qimen）.Rat models with liver-qi stagnation syndrome were established by chronic unpredicted mild stress and solitary feeding.Open-Field Test and sucrose water addiction test were carried out before and after experiment.Body weight and 24-hour food intake volume were measured one day before experiment,and 4,8,12,16,20,24,and 28 days after experiment.Results After experiment for 14 days,the body weight was increased slowly,and 24-hour food intake volume was decreased in the model group（P〈0.05 or P〈0.01 compared with those in the normal group）.After experiment for 20 days,the body weight and 24-hour food intake volume were increased in EA group（P〈0.05 compared with those in the model group）.On day 28 of the experiment,the scores of horizontal movement and vertical movement in Open-Field Test were reduced,and sucrose water consumption volume and the degree of sucrose water addiction were decreased in the model group as compared with those in the normal group（P〈0.05 or P〈0.01）,while EA had counteraction on the above indexes（P〈0.05 or P〈0.01 compared with the model group）.Conclusion EA on acupoints of Ganshu and Qimen exerts certain preventive and therapeutic effect on behavior changes in rats with stagnation of liver-qi.

关 键 词：肝气郁结证/针灸疗法 穴 肝俞 穴 期门 行为学 开野实验 蔗糖水消耗实验 疾病模型 动物 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]