化痰祛瘀法对腹膜纤维化大鼠TGF-β/Smad信号通路的影响  

Effect of Phlegm-resolving and Stasis-removing Therapy on TGF-β/Smad Signal Pathway in Peritoneal Fibrosis Rats

作　　者：陈刚毅[1] 苏保林[1] 孟立锋[1] 汤水福[1] 

机构地区：[1]广州中医药大学第一附属医院肾内科,广东广州510405

出　　处：《广州中医药大学学报》2012年第1期34-36,113,114,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局资助课题(编号:2008147)

摘　　要：【目的】探讨化痰祛瘀法对腹膜纤维化大鼠转化生长因子-β(TGF-β)/Smad信号通路的影响。【方法】选用健康SD雄性大鼠60只,随机分为5组,每组12只,分别为:空白组、模型组、西药组、中药高剂量组、中药低剂量组。复制大鼠腹膜纤维化模型后,即实验第5周开始,中药高、低剂量组分别给予化痰祛瘀中药50、25 mg.kg-1.d-1,西药组给予贝那普利5 mg.kg-1.d-1,模型组及空白组灌服注射用水,每天1次,共4周。实验第8周结束后留取腹膜组织,采用免疫组化法测定脏层腹膜p-Smad2/3、Smad7和TGF-β1表达情况。【结果】中药高剂量组、中药低剂量组、西药组Smad7表达均显著增加,与模型组比较差异均有统计学意义(P<0.05);中药高剂量组Smad2/3、TGF-β1表达显著降低,与中药低剂量组、西药组比较差异均有统计学意义(P<0.05);中药高剂量组Smad7表达显著升高,与中药低剂量组、西药组比较差异有统计学意义(P<0.05)。【结论】化痰祛瘀中药防治腹膜纤维化的作用与其能上调TGF-β抑制性信号蛋白Smad7的表达,抑制TGF-β受体调控信号蛋白Smad2/3表达有关。Objective To explore the effect of phlegm-resolving and stasis-removing therapy on tumor growth factor beta（TGF-β）/Smad signal pathway in peritoneal fibrosis rats.Methods Sixty healthy SD male rats were equally randomized into 5 groups： blank group,model group,western medicine group,high-and low-dose herbal medicine groups.Peritoneal fibrosis rats model was established firstly,and then the rats in the medication groups were given orally high-dose（50 mg·kg-1·d-1）or low-dose（25 mg·kg-1·d-1）herbal medicine with the actions of resolving phlegm and removing stasis,or benazepril（5 mg·kg-1·d-1） respectively.The rats in the model group and the blank group were given orally water for injection,once per day for 4 weeks.Peritoneal tissue was sampled at the end of the experiment.Immunohistochemical assay was used to examine the expression of phosphorylation-Smad2/3,Smad7 and TGF-β1 in the visceral peritoneum.Results Smad7 expression level was increased in high-,low-dose herbal medicine groups and benazepril group,the difference being significant compared with that in the model group（P〈0.05）.Smad2/3 and TGF-β1 expression levels were lower and Smad7 expression level was higher in high-dose herbal medicine group than those in low-dose herbal medicine group and benazepril group（P〈0.05）.Conclusion The preventive and therapeutic mechanism of phlegm-resolving and stasis-removing herbal medicine for peritoneal fibrosis is related with the up-regulation of TGF-β inhibitive signal protein Smad7 expression,and with the down-regulation of TGF-β receptor regulative signal protein Smad2/3 expression.

关 键 词：腹膜纤维化/中药疗法 化痰祛瘀法 TGF-Β/SMAD信号通路 腹膜/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]