液相色谱法测定对乙酰氨基酚片的含量  

Determination of Acetaminophen Pieces Content by HPLC

作　　者：石向群[1] 方莉[1] 徐秀景[2] 魏山青[1] 张扬[1] 

机构地区：[1]九江学院基础医学院,江西九江332000 [2]九江学院生命科学院,江西九江332000

出　　处：《广州化工》2012年第1期92-93,97,共3页GuangZhou Chemical Industry

摘　　要：建立了高效液相色谱法测定对乙酰氨基酚片中对乙酰氨基酚含量。色谱条件:C18(5μm,150 mm×4.6 mm),流动相为0.05 mol/mL醋酸铵溶液∶甲醇(85∶15),流速1 mL/min,检测波长为244 nm,柱温为30℃,对乙酰氨基酚在3～15μg/mL浓度范围内呈良好线性关系(r=0.9997)。该方法准确、可靠,与标准法比较,结果较为满意,可用于对乙酰氨基酚片中对乙酰氨基酚的含量测定。The method for determination of acetaminophen content in acetaminophen by HPLC was established.Chromatographic conditions were C18（5 μm,150×4.6 mm）,mobile phase of acetonitrile： 0.05 mol/mL,amm-onium acetate solution-methanol（85：15） at the flow rate of 1.0 mL/min,the detection wavelength 244 nm and the temperature of the column 30 ℃.The calibration curve of paracetamol was linear in the range of 3～15 μg/mL,r=0.9997.This method was accurate,realiable and can be used for the determination of acetaminophen content in ace-taminophen pieces.

关 键 词：高效液相色谱法 对乙酰氨基酚 

分 类 号：R927.11[医药卫生—药学]