大孔吸附树脂纯化木麻黄总黄酮及对青枯菌的抑制  

Purification of Total Flavonoids from Casuarina equisetifolia by Macroporous Adsorption Resin & Inhibition of Total Flavonoids on Ralstonia solanacearum

作　　者：王翠颖[1] 孙思[1,2] 周而勋[2] 王军[1] 

机构地区：[1]华南农业大学林学院,广东广州510642 [2]华南农业大学资源环境学院

出　　处：《防护林科技》2012年第1期11-14,32,共5页Protection Forest Science and Technology

基　　金：国家科技支撑计划课题(2009BADB2B0203-01)

摘　　要：为探索获得较为纯化的木麻黄总黄酮的便捷有效方法,研究了利用大孔吸附树脂分离纯化木麻黄总黄酮的工艺。结果表明:选择D101大孔吸附树脂的最佳工艺条件为吸附液料比20∶1(黄酮粗提液∶大孔吸附树脂,mL.g-1);吸附液pH为2;解吸液pH为11;最佳静置吸附时间为90 min;乙醇洗脱体积分数为80%;洗脱液料比为20∶1(80%乙醇溶液∶大孔吸附树脂,mL.g-1)。分离到的总黄酮对青枯菌具有明显的抑制作用。同时也证明了气质联用不适于鉴定黄酮类大分子物质。Process of separation purification of total flavonids from Casuarina equisetifolia by macroporous adsorption resin was studied in order to obtain pure total flavonids from Casuarina equisetifolia conveniently efficiently.Result shows that optimal process condition by selecting D101 macroporous adsorption resin is ： adsorption ratio（crude flavonoid extracts ： D101 macroporous adsorption resin,mL·g-1） is 20∶1;pH value of adsorption desorption solution are 2 11,respectively;optimal standing time is 90 min;percentages（%） of elution volume for ethanol are 80%;ratio of liquid to material is 20∶1（80 % ethanol： D101macroporous adsorption resin,mL·g-1）.The purified flavonoids have obvious inhibition effect on Ralstonia solanacearum.It also proved that gas chromatography-mass spectrometry is not suitable for analyze flavonoids.

关 键 词：木麻黄 青枯菌 黄酮 大孔吸附树脂 

分 类 号：S792.93[农业科学—林木遗传育种]