玉米抗大斑病菌单基因系的建立及其在Exserohilum turcicum生理小种鉴定中的应用  

Corn Resistant Single Gene Lines to Exserohilum turcicum and Their Application to Identification of Physiological Races of E.turcicum

作　　者：张桂珍[1] 刘金亮[1] 胡瑞学[1] Ali Khatib Bakar 张艳华[1] Don G.White 潘洪玉[1] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]美国伊利诺伊大学香槟分校作物科学系

出　　处：《吉林大学学报（理学版）》2012年第1期134-138,共5页Journal of Jilin University:Science Edition

基　　金：吉林省国际科技合作计划项目(批准号:20100723);长春市国际科技合作计划项目(批准号:10GH26);吉林大学研究生创新基金(批准号:20111078)

摘　　要：利用美国A632,B37,B73,B68,C103,Va26等30个Ht单基因鉴别寄主,按美国伊利诺伊大学的大田接种方案与鉴定方法,建立了适合中国的监测玉米大斑病菌生理小种鉴定的21个Ht单基因系.从吉林省15个地区的不同玉米品种中采集玉米大斑病样本,采用单孢分离获得了32个玉米大斑病菌菌株,利用已建立的上述21个Ht单基因鉴别寄主,通过美国的大田鉴定方案进行生理小种鉴定.结果表明,这32个菌株均为0号小种,并未出现生理小种分化现象.Thirty inbred lines such as A632,B37,B73,B68,C103 and Va26 from the inbred collection at the University of Illinois were used to determine physiological races of Exserohilum turcicum in the field.Twenty-one inbred lines from the inbred collection were selected to determine physiological differentiation of E.turcicum effectively in China.Thirty-two single conidial isolates of E.turcicum were collected throughout 15 farming areas of Jilin Province.All the 32 isolates were race zero.There was no physiological differentiation of the pathogen.

关 键 词：单基因系 玉米大斑病菌 生理小种 鉴定 

分 类 号：Q939.95[生物学—微生物学]