不同形态氮素对大豆硝酸还原酶和谷氨酰胺合成酶活性及蛋白质含量的影响  

Effect of different nitrogens on activities of nitrate reductase,glutamine synthetase,and seed protein contents in soybean cultivars

作　　者：胡润芳[1] 张广庆[1] 滕振勇[2] 林国强[1] 

机构地区：[1]福建省农业科学院作物研究所,福州350013 [2]福建省种子总站,福州350003

出　　处：《东北农业大学学报》2012年第1期31-35,共5页Journal of Northeast Agricultural University

基　　金：福建省农业科技重大专项项目(2008NZ0002-1(3));福建省财政专项(STIF-Y04);福建省科技厅公益类科研院所专项(2011R1028-3);农业部公益性行业(农业)科研专项(200903002)

摘　　要：利用硝态氮(NO3--N)、铵态氮(NH4+-N)和混合态氮对3个栽培大豆品种花荚期植株进行诱导处理,研究不同形态氮素对功能叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)活性以及籽粒蛋白质含量的影响。结果表明,NH4+-N增加3个大豆品种功能叶片NR活性效果最好,其次是混合态氮,NO3--N效果较差;三种形态的氮素均能明显提高3个大豆品种功能叶片GS活性;在三种形态氮素诱导下,3个大豆品种的籽粒蛋白质含量均有不同程度的提高,且与其功能叶片NR和GS活性呈显著正相关(r=0.520*和0.550*);追施氮素对低蛋白大豆品种功能叶片NR、GS活性和籽粒蛋白质含量具有较好的促进作用。可以把大豆花荚期叶片NR和GS的活性作为高蛋白品种选育的参考指标之一。Effects of different nitrogens （NO3-N, NH4＋-N, NO3--NH,＋） on nitrate reductase （NR） and glutamine synthetase （GS） activities of functional leaves, and seed protein contents of three soybean cultivars during flowering-poding phase were investigated. The results indicated that NR activities of functional leaves in three soybean genotypes treated by three kinds of nitrogens were enhanced, NH4＋-N was the best, the second was NO3-NH4＋, and the third was NO3-N. All three different nitrogen treatments could remarkably increase GS activities of functional leaves of three soybean cultivars. The seed protein content showed a very significantly positive correlation with the functional leaf NR activity and GS activity （r=0.520＊ and 0.550＊）. There is better promotion of nitrogen topdressing to activities of NR and GS in functional leaves and cotents of protein in seeds of the lower-protein soybean cultivar. Hence, it is suggested that high NR and GS activity in soybean functional leaves should be one of the bio-chemical index for selecting soybean germplasm with high protein.

关 键 词：大豆 硝酸还原酶 谷氨酰胺合成酶 蛋白质 

分 类 号：S565.1[农业科学—作物学]