RT-PCR法克隆广谱抗病基因TGA2及其植物表达载体构建  

Cloning of Arabidopsis thaliana broad-spectrum disease-resistant TGA2 gene and its plant expressing vector construction

作　　者：刘永光[1,2] 刘克锋[2] 孙向阳[1] 

机构地区：[1]北京林业大学水保学院,北京100083 [2]北京农学院城乡发展学院,北京102206

出　　处：《东北农业大学学报》2012年第1期137-142,共6页Journal of Northeast Agricultural University

基　　金：北京市教委面上项目(KM200910020014);北京市园林绿化局治沙办项目(2008)

摘　　要：文章采用Trizol Reagent提取拟南芥总RNA,设计相关引物;采用反转录PCR方法克隆TGA2转录因子,利用酶切连接方法,将该基因正向导入植物表达载体。经过相关检验,结果表明,TGA2转录因子正向插入中间载体的CaMV35S启动子和T-nos终止子之间,成功构建包含TGA2转录因子的p35ST-TGA2植物表达载体。After extracting the Arabidopsis thaliana RNA with Trizol Reagent and designing primes, TGA2 transcription factor gene was cloned by RT-PCR. In the study, the experiment inserts the TGA2 transcription factor between CaMV35S promoter and T-nos terminator of plant expression vector. The TGA2 transcription factor gene was correctly cloned into plant expression vectors. Therefore, it achieved the p35ST- NPR1 plant expression vectors containing TGA2 transcription factor.

关 键 词：TGA2转录因子 系统获得性抗性 广谱抗病 载体构建 

分 类 号：Q786[生物学—分子生物学]