正交试验法优化香柏(变种)中总黄酮提取工艺  

Optimization of Total Flavonoids Extraction from Sabina pingii(Cheng ex Ferre) Cheng et W.T. Wang var. wilsonii(Rehd.) Cheng et L.K. Fu Using the Orthogonal Test

作　　者：杨梅[1] 王文兰[1] 张嫦[1] 刘东[1] 王晓玲[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041

出　　处：《食品研究与开发》2012年第1期47-50,共4页Food Research and Development

基　　金：中央高校基本科研业务费专项资金(No:11NFW02)

摘　　要：研究提取香柏总黄酮的最佳工艺条件。以总黄酮的得率为考察指标,溶剂回流作为提取手段,利用单因素和正交试验,考察提取温度、提取时间、乙醇溶液体积分数、液固比以及提取次数对总黄酮得率的影响。最优工艺条件为:在80℃水浴条件下,提取时间90 min、乙醇溶液体积分数60%、液固比50∶1(mL/g)、提取次数3次。在最优工艺条件下总黄酮的提取率可达7.68 g/100 g。该方法操作方便、准确可靠,可以用于工业化生产。Determine the Cheng et W. T. Wang var optimal condition of extraction for total flavones in Sabina pingii （cheng ex Ferre） wilsonii（Rehd. ） Cheng et L. K. Fu. Extractions of Reflux was investigated. During the extracting process, five main factors including, extraction temperature, processing time, concentration of extracting solution, liquid-solid ratio, extraction times were studied by using single factor analysis method. Based on the single-factor experiment, the best conditions of extracting total flavones were optimized by orthogonal test. The optimums of reflux extraction were ： extracted in 80 ~C water bath, ethanol concentration 60 %, processing time 90 min; extraction times 3, liquid-solid ratio 50：1 （mL/g） ; under the above conditions, the total flavones yield 7.68 %. Those extraction methods were simple, rapid and applicable for the extraction of total flavones in Sabinapingii（cheng ex Ferre ） Cheng et W. T. Wang var. wilsonii（Rehd. ） Cheng et L. K. Fu.

关 键 词：香柏(变种) 总黄酮 回流提取 正交试验 

分 类 号：TQ461[化学工程—制药化工]