大鼠骨骼双染标本的制作方法  

The methods of the rat skeleton double stain andpreparation

作　　者：张宏[1] 李啸红[1] 

机构地区：[1]遵义医学院珠海校区人体解剖与组织胚胎学教研室,广东珠海519041

出　　处：《重庆医学》2012年第2期153-154,157,共3页Chongqing medicine

基　　金：贵州省科技厅科学技术基金项目[科合J字(2009)2215]

摘　　要：目的探讨大鼠骨骼双染方法,为制作教学陈列用标本或研究胚胎正常骨骼形态发育以及胚胎骨骼畸形提供基本的实验方法。方法将18d胎鼠及生后1d仔鼠去除皮肤、皮下脂肪组织及内脏,用阿利新蓝染液染软骨,再用含氢氧化钾的茜素红染液染骨,最后通过逐级甘油脱水、透明并保存。结果已骨化的骨被茜素红染成红色或紫红色,软骨被阿利新蓝染成蓝色,其他组织为无色透明状,在体视镜下可清楚地观察到骨骼的发育形态。结论采用茜素红和阿利新蓝双染法,可使去除皮肤、皮下脂肪组织及内脏的整体胎鼠和仔鼠骨和软骨获得最佳染色效果,是研究骨和软骨发育较为直观的方法,也是制作教学陈列标本的好方法。Objective To discuss the methods of rat skeleton double stain,in order to provide basic testing methods for making display samples for teaching or studying the normal skeleton morphological development and the skeletal deformity of embryos.Methods The 18 d fetal rat and filial rat 1 d after birth were removed skin,subcutaneous adipose tissue and viscera,and their cartilages were first dyed by the dye solution of Alcian blue,and then dyed by the dye solution of alizarin red containing potassium hydroxide,and finally dehydratsed by several levels of glycerin,made transparent and stored.Results The ossificated skeletons were dyed to red or amaranth by the alizarin red,the cartilages were dyed to blue by the Alcian blue,and other tissues were transparent.The development morphology of the skeleton could be clearly observed through stereoscope.Conclusion The dual dying method that adopts alizarin red Alcian blue could achieve the best dying effect on the skeletons and cartilages of complete fetal rats and filial rats whoes skins,subcutaneous adipose tissue and viscera had been removed,so it is a relatively direct method to study the development of skeletons and cartilages,and also a good method for making display samples for teaching.

关 键 词：大鼠 骨骼 软骨 双染法 注意事项 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R329-4[医药卫生—基础医学]