紫外分光光度法测定溴新斯的明-β-环糊精包合物  

Determination of Neostigmine Bromide-β-Cyclodextrin Inclusion Complex by UV Spectrophotometry

作　　者：李嫄[1] 谭群友[2] 邓波[2] 熊华蓉[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016 [2]第三军医大学大坪医院野战外科研究所胸外科

出　　处：《光谱实验室》2012年第1期371-374,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助.KJ090308)

摘　　要：采用紫外分光光度法测定溴新斯的明环糊精包合物中溴新斯的明含量。测定波长为260nm。溴新斯的明浓度在50-450pg·mL^(-1)范围内与吸光度呈良好的线性关系,回归方程为:A:0.0016C-0.0064,r=0.9999(n=3);平均回收率为100.0%(n=9).RSD为0.31%;日内RSD分别为1.06%、0.71%、0.86%(n=5);日间RSD分别为0.91%、0.61%、0.58%(n=3);测得3批样品的含量分别为4.83%、4.79%、4.71%。方法操作简便、迅速.具有良好的精密度,适用于溴新斯的明环糊精包合物中溴新斯的明含量的测定。The content of neostigmine bromide in neostigmine bromide-β-cyclodextrin inclusion complex was detected at the wavelength of 260nm by UV spectrophotometry.The neostigmine bromide had a good linear relationship in the range of 50—450μg · mL^（-1）with absorption.The regression equation was as follow：A = 0.0016C-0.0064,r= 0.9999（n = 3）.The average recovery was 100.0%（n= 9）,RSD=0.31%.The RSD of within day were 1.06%,0.71%and 0.86%（n = 5）,and RSD of between-day were 0.91%,0.61%and 0.58%（n = 3）.The contents of the three batches of samples were 4.83%,4.79%,4.71%.The method is simple,rapid and accurate,which can be used for the determination of the content of neostigmine bromide in neostigmine bromide-β-cyclodextrin inclusion complex.

关 键 词：溴新斯的明 紫外分光光度法 溴新斯的明-β-环糊精包合物 

分 类 号：O657.32[理学—分析化学]