西兰花种子提取萝卜硫素的酶解体系  

Enzymatic Degradation System for Sulforaphane Extraction from Broccoli Seeds

作　　者：吴华彰[1] 赵云利[2] 

机构地区：[1]蚌埠医学院生物科学系 [2]蚌埠医学院预防医学系

出　　处：《光谱实验室》2012年第1期431-434,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：安徽省高等学校省级教学研究项目(2008jyxm431);2011年度安徽高校自然科学研究项目(KJ2011Z252)

摘　　要：以提取萝卜硫素主要酶解因素:pH、催化缓冲液类型、Zn^(2+)浓度、Vc的浓度为4个考察因素,分别设立3个水平.采用L_9(3~4)正交试验设计,优化提取酶解体系。结果表明,各因素的重要性顺序为:pH>催化缓冲液类型>Ve的浓度>Zn^(2+)浓度。西兰花种子生产萝卜硫素的最佳条件为:pH 5.0、Zn^(2+)浓度为0.1mol/L、Vc的浓度为0.5mg/mL、以0.1mol/L醋酸一醋酸钠萝卜汁缓冲液为酶解缓冲体系。验证结果显示优选的缓冲液体系高效、稳定,可以作为西兰花种子提取萝卜硫素的酶解体系。In order to optimize the enzymatic degradation system for extracting sulforaphane from broccoli seeds,the L_9（3~4） orthogonal test was used to determine the factors of pH,type of catalysis buffer,content of Zn^（2＋） and Vc on the extraction solution,and each factor set 3 level.The important order of various factors obeyed as follows：pH type of catalysis buffer contentration of Vcconcentration of Zn^（2＋）.The optimum extraction conditions were pH of 5.0,0.1mol/L acetic acid-sodium acetate buffer solution with turnip juice containing 0.lmol/L Zn^（2＋） and 0.5mg/mL Vc. The extraction buffer solution is reasonable and feasible,and can be used as enzymatic degradation for sulforaphane extraction from broccoli seeds.

关 键 词：萝卜硫素 正交试验 西兰花种子 

分 类 号：R284.3[医药卫生—中药学] O657.72[医药卫生—中医学]