荷移分光光度法测定缬氨酸  

Determination of Valine by Charge-Transfer Spectrophotometry

作　　者：韩玲军[1] 李省云[1] 

机构地区：[1]太原师范学院(南区)化学系,太原市迎泽区黄陵路19号030031

出　　处：《光谱实验室》2012年第1期503-505,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(21176020);山西省高校科技开发项目(20080025)

摘　　要：用分光光度法研究缬氨酸与7,7,8,8-四氰基对苯醌二甲叉(TCNQ)的荷移反应。确定以pH=9.2的三酸缓冲溶液为介质,在50℃的水浴中加热30min,形成稳定的络合物,其λ_(max)=417nm,表观摩尔吸光系数ε=7.8×10~3L·mol^(-1)·cm^(-1),在1—9μg/mL的范围内符合比耳定律,回收率在96.7%—102.7%,相对标准偏差小于2.9%。The charge-transfer reaction of valine with 7,7,8,8-tetracyanoquinodimethane （TCNQ） was investigated by spectrophotometry.The experimental conditions were optimized and a new method for determination of valine has been developed.The stable charge-transfer complex was formed in Britton-Robinson buffer solution（pH=9.2） by heating in 50＇C water bath for 30min.The apparent molar absorptivity of the complexεwas determined for 7.8×10~3L · mol^（-1） · cm^（-1） at 417nm.Beer＇s Law was obeyed in the range of 1—9μg/mL of valine.The recovery was 96.7%—102.7%,and the relative standard deviation was less than 2.9%.

关 键 词：分光光度法 荷移反应 缬氨酸 7 7 8 8-四氰基对苯醌二甲叉 

分 类 号：O657.32[理学—分析化学]