NADPH氧化酶在大鼠心肌梗死后左室重构中的作用  

Effect of NADPH oxidase on left ventricular remodeling after myocardial infarction in rats

作　　者：朱俊国[1] 张林林[1] 李红霞[1] 赵彩明[1] 韩莲花[1] 李勋[1] 

机构地区：[1]苏州大学附属第一医院心脏病科,江苏省215006

出　　处：《江苏医药》2012年第1期4-6,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(81041041);苏州市科技计划(SYS201028)

摘　　要：目的探讨还原型辅酶Ⅱ(NADPH)氧化酶在大鼠心肌梗死后左室重构中的作用。方法 25只SD大鼠随机分为四组:心肌梗死组(A组,7只)、心肌梗死+夹竹桃麻素15mg.kg-1.d-1组(B组,6只)、假手术组(C组,6只)和假手术+生理盐水组(D组,6只)。6周后处死大鼠,称取心脏和左室重量,ELISA法检测胶原蛋白Ⅰ、Ⅲ含量,TUNEL法检测心肌细胞凋亡并分析其与左室射血分数(LVEF)的关系。结果与A组相比,B、C、D组心脏及左室重量指数、胶原蛋白Ⅰ含量及Ⅰ/Ⅲ比值、心肌细胞凋亡指数均明显降低(P<0.05或P<0.01),而LVEF则显著增高(P<0.01)。心肌细胞凋亡指数与LVEF呈负相关(r=-0.757,P<0.01)。结论心肌梗死后NADPH氧化酶激活可能是导致左室重构的重要原因之一。Objective To investigate the effect of NADPH oxidase on the left ventricular remodeling after myocardial infarction（MI） in rats. Methods Twenty-five SD rats were randomly divided into four groups of A（MI model,7 rats）,B（MI followed by injection of apocynin 15mg·kg-1·d-1,6 rats）,C（sham operated group,6 rats） and D（sham operated followed by equivalent normal saline, 6 rats）.All rats were sacrificed on the 6 th week after operation,and the heart and left ventricle were weighed.The contents of collagen Ⅰ and Ⅲ were detected by ELISA method. The apoptosis index of cardiomyocytes was measured by TUNEL and the correlation between the apoptosis index and the left ventricular ejection function（LVEF） was analyzed. Results Compared with group A,the heart and left ventricular mass indexes,the content of collagen Ⅰ,the ratio of collagen Ⅰ to collagen Ⅲ and the apoptosis index of cardiomyocytes were all significantly decreased（P0.05 or P0.01）,while the LVEF was obviously increased（P0.01） in groups of B,C and D.The apoptosis index of cardiomyocytes was negatively correlated with LVEF（r=-0.757,P0.01）. Conclusion The activation of NADPH oxidase may lead to left ventricular remodeling after MI.

关 键 词：还原型辅酶Ⅱ氧化酶 心肌梗死 心室重构 

分 类 号：R541[医药卫生—心血管疾病]