不同浓度甘油冷冻保护剂和冷冻麦管规格对七彩鲑受精率的影响  

Effects of Different Concentrations of Glycerol as Cryoprotectant and Different Sizes of Straw on Fertilization Rate of Salvelinus fontinalis

作　　者：邓双义[1] 李胜忠[1] 成沛[1] 关秀芝[1] 

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052

出　　处：《现代农业科技》2012年第1期293-293,296,共2页Modern Agricultural Science and Technology

基　　金：新疆科技计划项目(200941134)

摘　　要：以5%～30%浓度的甘油作为精液冷冻保护剂,以0.5 mL麦管和1.8 mL麦管作为精液冻存容器,进行七彩鲑冻精的受精试验。试验结果表明:20%浓度甘油组的受精率达到了51.08%～61.56%,显著高于其他浓度甘油组的受精率;在20%～25%甘油浓度中0.5 mL麦管组的受精率均高于1.8 mL麦管组的受精率,其中20%甘油的0.5 mL麦管组,平均受精率最高,达到了61.38%。The artificial fertilization experiment on Salvelinusfontinalis was carried out,using 5%-30% glycerol as eryoproteetant,and 0.5 mL and 1.8 mL straw as semen eryopreservation container. The results showed that：The fertilization rates of 20% glycerol group reached 51.08%-61.56%, which was obviously higher than other concentrations of glycerol groups. With the glycerol concentration of 20%-25% ,the fertilization rates of 0.5 mL straw group were significantly higher than the fertilization rates of 1.8 mL straw group. The mean fertilization rate of 20% glycerol and 0.5 mL straw group was the highest ,which reached 61.38%.

关 键 词：甘油 冷冻保护剂 冷冻麦管 七彩鲑 受精率 影响 

分 类 号：S961.2[农业科学—水产养殖]