白细胞介素-6介导抑郁模型大鼠结肠动力变化的机制研究  

Study on the mechanism of interleukin-6 in the colonic motility changes of depression rat model

作　　者：张丽[1] 陈明锴[1] 邓琴[1] 

机构地区：[1]武汉大学人民医院消化科,430060

出　　处：《中华消化杂志》2012年第1期33-36,共4页Chinese Journal of Digestion

基　　金：湖北省自然科学基金（2009CDB283）;武汉大学优秀青年教师基金（3082018）

摘　　要：目的研究慢性应激抑郁模型大鼠结肠动力变化、血清白细胞介素（IL）-6与抑郁的关系及对离体结肠肌条收缩的影响。方法健康成年Wistar大鼠30只，其中15只建立慢性应激抑郁模型，其余15只为对照组。以糖水偏爱实验检测两组大鼠的糖水消耗与体质量比、糖水偏爱百分比。以旷场实验检测两组大鼠的行为学变化。以模拟粪便排出实验评价结肠动力。以酶联免疫吸附测定（ELISA）检测血清IL-6水平。逆转录（RT）-PCR检测结肠组织中IL-6mRNA水平。检测大鼠离体结肠肌条的收缩功能。用不同浓度的IL-6处理对照组结肠肌条，检测IL-6对正常结肠肌条的影响及其对乙酰胆碱（Ach）的反应。结果模型组大鼠糖水消耗与体质量比为0．12±0．03、糖水偏爱百分比为（16．17±2．61）％、总行程为（741．54±341．10）cm、直立次数为（15．69±8．00）次，均显著低于对照组[0．18±0．02、（25．54±2．32）％、（1336．20±698．80）cm、（24．87±7．90）次，P值分别=0．041、0．044、0．002、0．001]。模型组大鼠粪便排出时间为（109．78±48．00）min，较对照组明显延长[（28．00±11．10）min，P=0．002]。模型组大鼠血清和结肠组织IL-6水平均较高。模型组离体结肠肌条对Ach反应弱于对照组（P=0．035）。对照组离体结肠肌条对200、500、1000pg／ml IL-6的反应差异均无统计学意义（P值分别=0．935、0．825和0．766），再加入Ach后，500和1000pg／ml I-L6组对Ach的反应（R值分别为1．15±0．10和1．14±0．15）与对照组单加Ach时的R值（1．61±0．45）比较，差异均有统计学意义（P值分别=0．000、0．004）。结论慢性应激抑郁模型大鼠血清及结肠组织IL-6水平上升，并通过降低肠道对Ach的敏感性而导致平滑肌收缩障碍。[Abstract] Objective To study the changes of colonic motility in chronic stress-induced depression rat model, the association between interleukin 6 （IL-6） and depression and its effects on contraction of colonic smooth muscle in vitro. Methods There were 30 healthy adult Wistar rats, of which chronic stress-induced depression model was created in 15 rats and the other 15 rats were in control group. The ratio of sugar consumption to body weight and the percentage of sugar preference were tested in these two rats groups through sugar preference experiments. The behavioral changes of two rats groups were detected by open field test. Colonic motility was evaluated with simulated fecal discharge experiment, and the serum IL-6 level was measured with enzyme linked immunosorbent assay （ELISA）. IL-6 in colon tissue at mRNA level was determined by RT-PCR. The contraction ability of rat colonic smooth muscle was observed. The colonic smooth muscle of control group was treated with different concentration of IL-6, the effects of IL6 on normal colonic smooth muscle and its response to acetylcholine （Ach） were evaluated. Results In model group, the ratio of sugar consumption to body weight was 0. 12±0. 03, the percentage of sugar preference was 16. 17%-±2.61%, total walking distance was （741.54±341.10） cm and total times of upright was 15.69＋ 8.00, which were all significantly lower than those of control group （0.18±0.02, 25.54±2.32%, 1336.20 cm±698.80 cm and 24.87±7.90, respectively） （P= 0. 041, 0. 044, 0. 002 and 0. 001）. It took （109. 78±48.00） min for defecating in model group, which was significantly longer than that of control group （28.00±11.10） min （P=0. 002）. The IL6 levels were high both in the serum and colon tissue of model group rats. The response of colonic smooth muscle to Aeh was weaker in model group than that of control group （P=0. 035）. In model group, there were no significant difference in the response of colonic smooth muscle to IL-6 at 200, 500 and 1000

关 键 词：应激 抑郁症 疾病模型 动物 结肠 动力学 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]