结核性胸膜炎患者胸液中CD4^+ CD25^+ FoxP3^+调节T细胞抑制抗结核免疫  

CD4^+ CD25^+ FoxP3^+ regulatory T cells suppressing cellular immune responses in tuberculous pleural effusion

作　　者：胡良安[1] 王晓虹 刘金华[1] 罗永艾[1] 

机构地区：[1]重庆医科大学附属第一医院肺科,重庆400016 [2]四川省成都市416医院

出　　处：《中国老年学杂志》2012年第1期17-19,共3页Chinese Journal of Gerontology

基　　金：重庆市自然科学基金资助项目(CSTC2007BB5293)

摘　　要：目的研究结核性胸膜炎患者胸液中CD4+CD25+FoxP3+调节T细胞是否增多,这些调节T细胞是否抑制结核的特异细胞免疫反应。方法使用细胞分离、流式细胞分析及体外细胞培养作细胞增殖及增殖抑制等实验方法,对15例结核性胸膜炎患者及17例健康正常人群胸液及外周血白细胞中CD4+CD25+FoxP3+调节T细胞的量及特征作研究。结果结核性胸膜炎患者胸液中CD4+CD25+FoxP3+调节T细胞明显高于患者及健康人群外周血。在体外,结核性胸膜炎患者胸液中单核细胞对BCG刺激产生γ-干扰素(IFN-γ)的能力明显强于患者及健康人群外周血中单核细胞;把这些调节T细胞从胸液单核细胞中清除,增强了结核患者胸液单核细胞对BCG刺激产生IFN-γ;从结核患者胸液分离的这些调节T细胞能抑制结核患者Th1细胞产生IFN-γ。结论结核性胸膜炎患者胸液CD4+CD25+FoxP3+调节T细胞增多,抑制结核性胸膜炎患者Th1细胞免疫反应,从而参与了结核性胸膜炎的发病。Objective To determine whether CD4^＋ CD25^＋ FoxP3^＋ regulatory T cells are increased in tuberculous pleural effusion （TPE） and suppress cellular immune responses. Methods The frequency and the function of regulatory T cells from pleural fluid and blood in 15 untreated TPE patients and circulating regulatory T ceils in 17 healthy control subjects were compared by using cell separation, flow cytometry analysis, proliferation assays and cytokines determination. Results The number of CD4^＋ CD25^＋ FoxP3^＋ regulatory T ceils significantly were increased in the pleural fluid than those of peripheral blood from TPE patient and healthy control subjects. The pleural fluid mononuclear cells （PFMCs） from TPE had significantly higher IFN-γproduction in response to BCG compared to peripheral blood mononuclear cells （PBMCs） from both TPE patient and healthy donors. Depletion of CD4^＋ CD25^＋ FoxP3^＋ regulatory T ceils from PFMCs of TPE patient enhanced BCG-induced IFN-γproduction. These CD4^＋ CD25^＋ FoxP3^＋ regulatory T lymphocytes isolated from the pleural fluid were capable of suppressing BCG-stimulated IFN-γ production in TPE patients. Conclusions CD4^＋ CD25^＋ FoxP3^＋ regulatory T cells are increased in patients with TPE, suppress Thγ-type immune responses and may therefore contribute to the pathogenesis of human tuberculosis.

关 键 词：CD4+CD25+FoxP3+调节T细胞 结核 胸膜炎 结核菌免疫 

分 类 号：I21[文学—中国文学]