婴幼儿腹泻A群轮状病毒G和P的基因分型研究  

Molecular genotyping analysis of group A rotavirus in children

作　　者：杨爱平[1] 汪敏[1] 许文龙[1] 张国祥[1] 

机构地区：[1]浙江萧山医院检验科,浙江杭州325000

出　　处：《中国微生态学杂志》2012年第1期73-75,共3页Chinese Journal of Microecology

摘　　要：目的研究浙江萧山医院婴幼儿童腹泻标本中人轮状病毒(Human Rotavirus)毒株的感染情况及G和P基因型流行特点。方法收集该院2009年8月至2010年8月腹泻儿童15233份粪便标本采用酶联免疫吸附试验、逆转录-巢式聚合酶链反应进行轮状病毒病原检测,将128份阳性标本进行VP7和VP4基本分型。结果 15233份婴幼儿腹泻标本中有2 706份标本为轮状病毒阳性,阳性率17.8%;男孩和女孩检出率差异无统计学意义,以6～12月龄段检出率最高;对128份阳性标本进行G血清分型和P基因分型,G1型53份(41.4%)、G3型38份(29.7%)、G1G3型17份(13.3%)、G未分型20份(15.6%);P[8]型72份(56.3%)、P[4]型16份(12.5%)、P[8]P[4]型3份(2.3%)、P未分型37份(28.9%),G血清型和P基因型的组合以G1P[8]为主,占29.7%(38/128)。结论浙江萧山医院A群轮状病毒G血清以G1型为主,其次为G3型,P基因型以P[8]型为主。Objective To analyze the epidemic characteristics of group A rotavirus in children by serotyping G and genotyping P. Method 15 233 samples of patients with diarrhea under 5years from August 2009 to August 2010 were collected for detection of rotavirus. The 128 rotavirus antigen-positive stool specimens were enrolled for serotyping G and genotyping P by RT-Nest PCR. Result The rotavirus existed in 17.7% （2706/15233） of the stool specimens. There was no significant differences between genders. The highest detectable rate exsited in children aged 6-12 month. Of the total 128 G serotyping, G1 accounted for 41.4%, G3 29.7%, G1 G3 13.3% and non-typeable 15.6%. 72 （56.3%） of the strains were P [ 8 ], fol- lowed by 16 （ 12.5 % ） P [ 4 ], and a total of 38 （29.7%） isolates were found to be non-typeable. G1P [ 8 ] was the predominant combination, with the proportion of 29.7%. Conclusion G1 and P [ 8 ] are the most common serotype and genotype of group A rotavirus in our hospital.

关 键 词：轮状病毒 腹泻 分子流行病学 

分 类 号：R373.25[医药卫生—病原生物学]