整合素α_vβ_3和VEGF在骨折愈合过程中的表达及意义  

Expression and significance of integrin α_vβ_3 and VEGF in the fracture healing

作　　者：杨庆秋 胡侦明[2] 浦波[3] 劳汉昌[3] 

机构地区：[1]云南省第三人民医院骨科,650011 [2]重庆医科大学附属第一医院骨科,400016 [3]昆明医学院第二附属医院骨科,650101

出　　处：《中国矫形外科杂志》2012年第2期165-168,共4页Orthopedic Journal of China

基　　金：云南省自然科学基金资助项目(编号:2004C0068M)

摘　　要：[目的]探讨整合素αvβ3和VEGF在骨折愈合过程中的表达及意义。[方法]将新西兰大白兔20只,随机分为4组,每组5只,手术制作左侧兔桡骨中段骨干缺损模型,作为实验组。右侧作假手术处理,作为对照组;应用免疫组织化学链霉亲合素-生物素酶复合物(SABC)法检测术后2、4、8、12周骨痂组织中整合素αvβ3和VEGF的表达。[结果]在骨折愈合的不同时期,整合素αvβ3和VEGF的表达上调,对照组与实验组的表达差异有统计学意义(P<0.05)。术后2周整合素αvβ3和VEGF的表达水平最高,术后12周表达水平最低。[结论]整合素αvβ3和VEGF可能在骨折愈合的过程中起重要作用,检测整合素αvβ3和VEGF的表达有助于对骨折预后的判断。[ Objective ] To study the expression of integrin αvβ3 and VEGF in the fracture healing, and to evaluate their significance. [ Method ] Twenty New Zealand rabbits were randomly divided into 4 groups in this experiment, and each group has five rabbits. The fracture model of left radius was established by operation （ experiment group） and right radius by sham opera- tion （control group）. Using streptavidin biotin-peroxidase complex （SABC） method, we studied the expression of integrin αvβ3 and VEGF in normal bone and callus tissue at 2,4,8, and 12 weeks after operation. [ Result ] The expression of integrin αvβ3 and VEGF protein was increased in callus compared to the normal. The differences were statistically significant （ P 〈 0.05 ）. The highest expression levels of integrin αvβ3 occurred at 2 weeks after operation, and the lowest at 12 weeks after operation. [ Conclusionl The integrin αvβ3 might play an important role in the fracture healing. The detection of these proteins might contribute to the prognosis of fracture healing.

关 键 词：整合素ΑVΒ3 VEGF 骨折愈合 

分 类 号：R274.1[医药卫生—中医骨伤科学] R683[医药卫生—中医学]