眼针对脑缺血再灌注模型大鼠ERK1/2信号转导通路影响的实验研究  

The Experimental Study On Eye-acupuncture Effect Upon ERK1/2 Signaling Pathway of Cerebral Ischemia Reperfusion Rat Model

作　　者：张威[1] 马贤德[1] 王健[1] 

机构地区：[1]辽宁中医药大学附属医院神经内科,沈阳110032

出　　处：《中国医科大学学报》2012年第1期42-44,共3页Journal of China Medical University

基　　金：国家重点基础研究发展计划项目(973计划)资助项目(2007CB512702);辽宁省教育厅高校科研计划(L2010351);沈阳市科技计划项目(F11-264-1-6)

摘　　要：目的探索脑缺血再灌注损伤后ERK1/2信号转导通路的激活情况,研究眼针疗法对该通路的影响。方法应用线栓法复制出脑缺血再灌注损伤大鼠的模型,采用眼针治疗。再灌注24 h后处死取材,采用Western blot方法对各组大鼠海马组织中ERK1/2及p-ERK1/2的表达进行检测。结果眼针疗法能够显著上调脑缺血再灌注损伤后大鼠海马组织中ERK1/2、p-ERK1/2蛋白的表达,与模型组比较,有统计学意义。结论眼针疗法能够显著上调脑缺血再灌注模型大鼠ERK1/2及p-ERK1/2的表达;眼针疗法的脑保护作用可能通过激活ERK1/2信号转导通路而实现。Objective To explore ERK1/2 signaling pathway activation after cerebral ischemia reperfusion injury,to study the effect of eyeacupuncture therapy on the ERK1/2 pathway. Methods string thrombus method was used to replicate the model of cerebral ischemia reperfusion injury tat, eye-acupuncture therapy was applied thereafter. After reperfusing 24 hours ,the rats was killed ,the ERK1/2 and p- ERK1/2 expression in the rat＇s hippocampus were detected by Western-blot method. Results The reduced expression level of ERK1/2,p- ERK1/2 protein in hippocampus of cerebral ischemia- reperfusion injury rat was improved through adopting eye acupuncture therapy,compared with model group. Conclusion Eye-acupuncture therapy could significantly improve the expression level of ERK1/2 and p-ERK1/2 restrained by cerebral ischemia-reperfusion injury in rat hippocampus;eye-acupuneture therapy could make cerebral protected through activating ERK1/2 signaling pathway.

关 键 词：眼针 脑缺血再灌注 ERK1/2 P-ERK1/2 

分 类 号：R245.319[医药卫生—针灸推拿学]