羧甲基甲壳素/海藻酸钠水凝胶溶胀性能及蛋白质控释性能研究  

Swelling Property of Carboxymethyl Chitin/Sodium Alginate Hydrogel and Application in Protein Controlled Release Systems

作　　者：高远[1] 李蕊[1] 郑翠芬[1] 周珊[1] 孙立苹[1] 

机构地区：[1]浙江农林大学环境与资源学院,浙江临安311300

出　　处：《安徽农业科学》2012年第3期1649-1651,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省科技厅项目(2010c33070);浙江农林大学科技创新项目(100320)

摘　　要：将羧甲基甲壳素与海藻酸钠共混制备复合水凝胶,并探讨了pH、海藻酸钠与羧甲基甲壳素的共混比例、海藻酸钠浓度、交联剂用量等因素对其溶胀性能的影响。结果表明,当两者的共混比例为4∶6、海藻酸钠浓度为4.5%、羧甲基壳聚糖浓度为5.0%、交联剂用量为0.30时,水凝胶的溶胀率较大;在pH=1.2时,BSA的释放率为5.60%,而当pH=7.4时,BSA的累积释放率达到79.80%。该水凝胶能在胃部的酸性环境中保护大分子药物,从而将其运载到高pH的肠道中并释放,显示出用于口服药物缓释体系的应用潜力。The carboxymethyl chitin and sodium alginate（SA） were mixed to prepare hydrogel.The effects of pH value,the proportion of SA to carboxymethyl chitin,the amount of SA and the dosage of crosslinker on the swelling behaviors of hydrogels have been studied.The results showed that the factors related above regulated their swelling behavior.The swelling ratio（SR） of hydrogel was the larger one at the condition in which the proportion of SA to carboxymethyl chitin,the concentration of SA,the concentration of carboxymethyl chitin and the dosage of crosslinker were 4∶6,4.5%,5.0% and 0.30 separately.The release behavior of Bovine Serum Albumin（BSA） entrapped in the hydrogels was of distinctly difference with the changes of pH value of loading medium.The release ratio of BSA in CMCT hydrogel in the medium of pH = 1.2 was 5.6% while in the medium of pH=7.4,the total release ratio was 79.8%.The hydrogel could protect macromolecular drugs in the sour environment of stomach,then,carried it into intestinal tract with high pH,finally,released,which showed the application potential in oral drugs controlled-release system.

关 键 词：羧甲基甲壳素 海藻酸钠 水凝胶 溶胀率 控制释放 

分 类 号：Q946.3[生物学—植物学]