对活性污泥中6种内分泌干扰物的定量测定  

Quantitative determination of six endocrine disrupting compounds in activated sludges by ASE-SPE-GC-MS

作　　者：叶欣[1] 张娴[1] 张晗[1] 陈少华[1] 王宁[2] 张丹丹[1] 

机构地区：[1]中国科学院城市环境研究所城市环境与健康重点实验室,厦门361021 [2]山东大学环境研究院,济南250100

出　　处：《环境化学》2012年第1期100-106,共7页Environmental Chemistry

基　　金：国家水专项项目子课题(2008ZX07315-003-02);厦门市科技计划项目(3502Z20100134)资助

摘　　要：针对活性污泥中6种内分泌干扰物(Endocrine Disrupting Compounds,EDCs):双酚A(BPA),雌酮(E1),17β-雌二醇(E2),雌三醇(E3),17α-乙炔雌二醇(EE2)和己烯雌酚(DES),建立了快速溶剂萃取(Accelerated Solvent Extractor,ASE),固相萃取(SPE)净化,GC-MS检测的测试方法.对比分析了不同衍生化试剂、衍生化条件对分析结果的影响,同时优化了净化步骤.通过所建立的方法对6种EDCs在污泥中的浓度进行分析,检测回收率为77.2%—118.3%,相对标准偏差小于9%.应用该方法测定了重庆某2个污水处理厂剩余污泥中的EDCs浓度.A sensitive method has been developed for simultaneous determination of six endocrine disrupting compounds,namely bisphenol A（BPA）,estrone（E1）,17β-estradiol（E2）,estriol（E3）,17α-ethynylestradiol（EE2） and diethylstilbestrol（DES） in activated sludges.Samples were extracted by accelerated solvent extractor（ASE）,followed by SPE clean-up,and detected by GC-MS.Different derivatization reagents and conditions were compared,then the clean-up procedure was optimized.The developed method showed good recoveries ranging from 77.2% to 118.3% with relative standard deviations within 9%.Finally,the established method was applied to determine the level of EDCs in excess sludge from two WWTPs located in Chong Qing.

关 键 词：内分泌干扰物 污泥 快速溶剂萃取 净化 

分 类 号：X503.1[环境科学与工程—环境工程]