精浆抗精子抗体影响精子DNA完整率的临床观察  

Effects of semen antisperm antibody on human sperm DNA activity

作　　者：王英俊[1] 李大文[2] 

机构地区：[1]重庆医科大学附属第二医院泌尿外科,400010 [2]广西壮族自治区人民医院遗传与生殖中心,南宁530021

出　　处：《免疫学杂志》2012年第2期177-180,184,共5页Immunological Journal

基　　金：广西医疗卫生重点科研课题(重200806)

摘　　要：目的研究精浆抗精子抗体(AsAb)对精子DNA完整率的影响。方法实验前取配偶怀孕后半年内正常精液标本20例测定精子DNA完整率做预试验,其数值拟做疗效参考指标。选择2008年4月至2011年1月来我院生殖中心就诊的特发性弱精子症门诊患者432例。检验其精浆抗精子抗体和精子DNA完整率。结果在432例病人中抗精子抗体阳性患者有45例,占总数的10.42%。抗精子抗体阳性组与正常组比较,在液化时间、精子密度、精子存活率、DFI(%()精子DNA断裂指数)上存在差异,具有统计学意义(P<0.05)。抗精子抗体阳性组与阴性组比较,在液化时间、精子密度、和排精量上存在差异,具有统计学意义(P<0.05)。抗精子抗体阳性组与阴性组比较,在精液pH值、DFI上无明显差异,无统计学意义(P>0.05)。结论精浆抗精子抗体(AsAb)对精子DNA完整率的影响不明显。Infertile men have poorer sperm DNA integrity than do fertile men,and this damage may contribute to reducing male reproductive potential.However,the etiology of this damage has not been fully characterized.We sought to examine the relationship,if any,between anti-sperm antibodies and sperm DNA damage in a consecutive series of non-azoospermic,infertile men in order to determine whether anti-sperm antibodies（AsAb） contribute to sperm DNA damage.The integrity of sperm DNA was determined in 20 cases of normal sperm samples and the parameters are treated as references.Total of 432 cases with idiopathic asthenospermia recruited from the reproductive center of our hospital from April 2008 to January 2011.AsAb and sperm DNA activity of all patients’ were measured.AsAb positive rate was 10.42% in 432 case of male infertility.In ASAb positive cases,sperm density and viability were lower,semen liquefaction was longer,and DNA fragmentation index（DFI） were more as compared with normal fertile man（P 〈 0.05）.The comparison between AsAb positive group and AsAb negative group in infertility cases also displayed significant differences of sperm density,semen liquefaction and the volume of semen（P 〈 0.05）,but no significant difference of DFI and pH of semen（P 〉0.05）.In conclusion,antisperm antibody might not affect human sperm DNA activity.

关 键 词：男性不育 抗精子抗体 精子DNA完整率 

分 类 号：R698.2[医药卫生—泌尿科学]