不同结构类型糖类物质的荧光标记策略(英文)  

Fluorescence Labeling Strategies for Different Structural Carbohydrates

作　　者：孙淑萌[1] 赵峡[1] 于广利[1] 李广生[1] 

机构地区：[1]中国海洋大学医药学院,教育部海洋药物重点实验室,山东省糖科学与糖工程重点实验室,山东青岛266003

出　　处：《中国生物化学与分子生物学报》2012年第1期18-27,共10页Chinese Journal of Biochemistry and Molecular Biology

基　　金：Supported by National Natural Science Foundation of China(No.30870506,No.31070724);Qingdao Science and Technology Project(No.11-2-2-1-hy);Public Science and Technology Research Funds Projects of Ocean(No.201005024)~~

摘　　要：为阐明糖链结构与功能的关系,寻找高灵敏度和高分离度的微量分析方法对糖类物质的分析至关重要.近几年来,荧光标记方法作为糖类物质的色谱定量及辅助结构分析的最佳选择,日益受到人们的广泛关注.荧光标记法分为柱前标记和柱后标记两种,且灵敏度高、分离度好、有多种标记试剂可供选择.本文根据不同糖类物质的结构特点,分别对中性糖和氨基糖以及含有唾液酸、糖醛酸、硫酸基的酸性糖的荧光标记方法及其应用进行了系统的综述,并对近年来报道的荧光标记试剂的特点和反应机理进行了比较和总结,以期为糖类物质微量分析方法的研究提供参考.Carbohydrates have become a hotspot in the field of glycoscience due to their various bioactivities and important biological roles in the life activities.High sensitivity and high resolution microanalytical techniques are required to elucidate the relationship between carbohydrate structure and their complicate biological functions.In recent years,several analytical technologies of labeling methods have been developed.The fluorescence labeling methods which improved the chromatographic determination and structural characterization have drawn wide attention.Both pre-column and post-column approaches have shown several advantages of high sensitivity,good resolution and versatility for labels.This paper compares the features and mechanisms of various fluorescent derivatization methods in applications for different carbohydrates of neutral,amino-,and acidic sugars,including sialic acid,uronic acid and sulfated sugars,as well as non-reducing sugars.

关 键 词：中性糖 氨基糖 唾液酸 糖醛酸 荧光标记 

分 类 号：Q53[生物学—生物化学] Q71