小续命汤有效成分对慢性脑缺血大鼠学习记忆能力及病理损伤的影响  

Effects of the active components of Chinese herbal medicine Xiaoxuming Decoction on memory behavior and brain injury in rats with chronic cerebral ischemia

作　　者：王月华[1] 贺晓丽[1] 杨海光[1] 秦海林[1] 杜冠华[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《中西医结合学报》2012年第1期91-99,共9页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金重点项目(No.30630073);北京协和医学院2011协和青年科研基金

摘　　要：目的:观察小续命汤有效成分对大鼠慢性脑缺血损伤的保护作用。方法:采用双侧颈总动脉永久性结扎法建立慢性脑缺血大鼠模型,将造模成功的大鼠分为模型组、银杏叶提取物组和低、中、高剂量小续命汤有效成分组。另设假手术组作为对照。Morris水迷宫法检测大鼠学习记忆能力,激光多普勒血流仪测定脑组织血流量及脑血管反应性,Nissel染色观察海马CA1区及大脑皮层的神经元结构,Klüver-Barrera染色观察脑白质变化,免疫组织化学染色观察脑组织星形胶质细胞的活化情况。结果:小续命汤有效成分可显著改善慢性脑缺血导致的大鼠认知能力下降。与慢性脑缺血模型组大鼠比较,小续命汤有效成分组大鼠在训练期和空间探索实验中到达平台的时间均缩短,中剂量组尤为明显(P<0.05);低剂量和中剂量小续命汤有效成分对慢性脑缺血大鼠脑血管储备功能降低具有显著的改善作用(P<0.05);各剂量小续命汤有效成分还可显著改善慢性脑缺血导致的脑部损伤,改善慢性脑缺血引起的神经元数目减少及形态和分布的异常;低剂量和中剂量小续命汤有效成分可显著减轻慢性脑缺血引起的脑白质病变(P<0.05,P<0.01);高、中、低剂量小续命汤有效成分均可降低星形胶质细胞的活化。结论:小续命汤有效成分对慢性脑缺血损伤具有显著的改善作用。OBJECTIVE： To study the effects of the active components of Xiaoxuming Decoction （XXM）, a compound traditional Chinese herbal medicine, on chronic cerebral ischemia in rats. METHODS： Chronic cerebral ischemia was induced in rats by occlusion of bilateral common carotid arteries. Then, the rats with chronic cerebral ischemia were randomly divided into five groups： model group, extract of Ginkgo biloba group and low-, medium- and high-dose active components of XXM groups. Another 11 rats without occlusion of common carotid arteries were used as the sham-operation group. Memory behavior was investigated by Morris water maze test. The structure of hippocampus and cortex neurons was observed with Nissel staining. The white matter lesion was stained with Klüver-Barrera stain method to observe the pathological changes. The astrocyte activation was observed using immunohistochemical method with glial fibrillary acidic protein （GFAP） antibody. RESULTS： The active components of XXM could significantly improve the impairment of learning and memory induced by chronic cerebral ischemia in rats. Compared with the model group, the time to reach the platform for rats was shortened by treating with the active components of XXM in Morris water maze test, particularly in the medium-dose group （P〈0.05）. In addition, the low- and medium-dose active components of XXM improved the decrease of cerebrovascular reactivity induced by chronic cerebral ischemia. The results of the pathological analysis also suggested that the active components of XXM could ameliorate the pathological damage induced by chronic cerebral ischemia in rats with the number of neurons increased, and the morphology and distribution of neurons recovered to normal levels. The low-dose active components of XXM significantly reduced the white matter lesions （P〈0.05, P〈0.01）. Active components of XXM treatment could also reduce the activation of astrocytes. CONCLUSION： The active components of XXM may attenuate the chronic cerebral ischem

关 键 词：植物提取物 缺氧缺血 脑 病理 行为研究 神经胶质原纤维酸性蛋白质 大鼠 

分 类 号：R285.5[医药卫生—中药学]