海南岛巴西橡胶和香蕉cat相关基因的克隆及分析  

Cloning and Analysis of the cat Associated Genes of Hevea brasiliensis and Banana in Hainan

作　　者：余乃通[1,2] 孙玉娟[2] 张雨良[2] 罗志文[1] 刘志昕[2] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《植物研究》2012年第1期47-53,共7页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31070131);海南大学“211工程大学建设”专项资金

摘　　要：根据GenBank上已有的植物cat基因序列,设计一对兼并引物。在海南岛采集香蕉、巴西橡胶、黄灯笼辣椒、菠萝、甘蔗、番木瓜等作物的叶片并提取总RNA,反转录成cDNA,PCR进行同源克隆。结果获得了巴西橡胶cat-1和香蕉的cat-2基因,而未获得其它4种作物cat相关基因。序列分析发现,巴西橡胶cat-1和香蕉的cat-2基因开放阅读框(ORF)都为1 479 bp,编码492个氨基酸,GenBank登陆号分别为HQ660587和HQ660588。生物信息学软件分析巴西橡胶CAT-1和香蕉CAT-2蛋白的三级结构分别与Exiguobacteriu Oxidotolerans(PDB code:2j2mA0)和Pseudomonas Syringae(PDB code:1m7sA)相似。构建系统发育树表明巴西橡胶CAT-1与蓖麻CAT-1氨基酸序列(Ricinus communis:XP_002521709.1)同源性最高,为92.1%;香蕉CAT-2与油棕CAT-2氨基酸序列(Elaeisguineensis:ACF06566.1)同源性最高,达90.9%。According to the existence of plant cat genes in GenBank,we designed a pair of degenerate primers,and amplified cat genes of banana,Hevea brasiliensis,yellow lantern pepper,pineapple,sugar cane,papaya from Hainan island.Ultimately,we cloned the rubber cat-1 and banana cat-2 genes,while the others not.Sequence analysis showed that open reading frame（ORF） of rubber cat-1 and banana cat-2 genes were both 1479bp,and encoded 492 amino acids.GenBank accession number were HQ660587 and HQ660588.Using bioinformatics software,the tertiary structures of rubber＇s CAT-1 and banana＇s CAT-2 proteins were predicated,which were similar to Exiguobacteriu Oxidotolerans（PDB code：2j2mA0） and Pseudomonas Syringae（PDB code：1m7sA）,respectively.Phylogenetic analysis indicated that Hainan H.brasiliensis CAT-1 possessed the highest homology with Ricinus communis CAT-1（XP_002521709.1）,while banana CAT-2 was the highest homology with Elaeis guineensis CAT-2（ACF06566.1）.

关 键 词：香蕉 巴西橡胶 CAT基因 克隆 序列分析 

分 类 号：S668.1[农业科学—果树学] S794.1[农业科学—园艺学]