芍药组织培养中污染现象的克服  

Contamination Control during Tissue Culture of Peony

作　　者：王吉凤[1] 李青[1] 包欣[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术中心,北京100083

出　　处：《植物研究》2012年第1期84-90,共7页Bulletin of Botanical Research

基　　金：十一五国家科技支撑计划项目(2006BAD07B01)

摘　　要：针对芍药在组织培养过程中外植体污染难于控制的问题进行了研究。试验分别于不同的季节取材后对外植体进行不同的表面消毒处理。结果表明:以芍药带芽茎段、顶芽、侧芽为外植体,采用0.1%～0.2%HgCl2消毒8 min,结合母株黄化处理、4℃消毒液+搅拌的表面消毒处理,可降低外植体初期培养污染率;在培养基中添加抗菌素,可明显控制内生菌的污染。To solve the problem of explant contamination during peony tissue culture,the explants of different seasons were studied.The results showed that soaking explants in 0.1%-0.2% HgCl_2 for 8min,etiolation treatment,low temperature and electronic vibration are effective for sterilization;Antibiotics added into the media can inhibit contamination caused by endophytes.

关 键 词：芍药 外植体 组织培养 污染 抗菌素 

分 类 号：S682.12[农业科学—观赏园艺]