不同年龄Ⅱ型糖尿病患者中黑白头发mtDNA异质性研究  

Heterogeneity of mitochondrial DNA in black and white hair of patients with type 2 diabetes

作　　者：谭凤明[1] 程喜平[1] 陈盛强[2] 陈智超 王延平[2] 沈岩松[2] 

机构地区：[1]广州医学院第一附属医院皮肤科,广东广州510120 [2]广州医学院第二附属医院神经科学研究所,广东广州510260 [3]广州市老人院,广东广州510550

出　　处：《南方医科大学学报》2012年第1期85-88,共4页Journal of Southern Medical University

基　　金：广东省科技计划(2010B031600303)

摘　　要：研究T2DM患者头发中mtDNA异质性的规律。 方法 选择T2DM高突变位点的mtDNA编码区和控制区2片段进行PCR扩增，以 DHPLC技术分析黑、白头发mtDNA目标片段在T2DM组和对照组异质性差异。结果 在20~45岁对照组、20~ 45岁T2DM组、45~70岁对照组和45~70岁T2DM组4个研究对象群，黑头发的mtDNA异质性分别为：3%，10%，9%，17%，白头发的mtDNA异质性分别为：9%，20%，21%，40%。其中同年龄T2DM组和对照组比较：20~45岁、45~70岁2个年龄组T2DM患者黑头发mtDNA异质性均高于同年龄对照组，45~70岁T2DM组白头发mtDNA异质性也高于同年龄对照组患者；不同年龄的 T2DM组以及不同年龄对照组之间比较：45~70岁对照组白头发mtDNA异质性高于20~45岁对照组、45~70岁T2DM组黑头发 mtDNA 异质性明显高于 20~45 岁 T2DM 患者（P〈0.05） ；组内比较：白头发 mtDNA 异质性在 45~70 岁对照者和 2 个年龄组的 T2DM患者组内内明显高于黑头发（P〈0.05）。 结论 T2DM患者mtDNA异质性增高，年龄越大程度越高。Objective To detect the heterogeneity of mitochondrial DNA（mtDNA） in black and white hair of patients with type 2 diabetes.Methods MtDNA was extracted from the hair shaft of the patients to amplify two target DNA fragment from mtDNA coding region and control region using PCR.The differences in the heterogeneity in the target DNA fragment was analyzed between diabetic patients and the control group with denaturing high-performance liquid chromatography（DHPLC）.Results In the control subjects and diabetic patients,the mtDNA heterogeneity in the black hair was 3% and 10% in 20-45 year-old groups and 9% and 17% in 45-70 year-old groups,as compared to 9%,20%,21%,and 40% in the white hair,respectively.The mtDNA heterogeneity in the black and white hair was both higher in the diabetic patients than in the control subjects of the same age group,and was also higher in older age subgroups in both control and diabetic groups（P0.05）.The white hair mtDNA showed a significantly higher heterogeneity than the black hair mtDNA in the two age groups of diabetic patients and in 45-70 year-old control group（P0.05）.Conclusion The mtDNA heterogeneity in the hair increases in type 2 diabetic patients and show an association with aging.

关 键 词：头发 2型糖尿病 MTDNA 异质性 变性高效液相色谱 

分 类 号：R587.1[医药卫生—内分泌]