汉防己甲素对人眼Tenon囊成纤维细胞中bax、bcl-2、TGF-β2 mRNA表达的影响  

Effect of tetrandrine on bax,bcl-2 and TGF-β2 mRNA expressions in cultured human Tenon's capsule fibroblasts

作　　者：李岱[1,2] 孙明[1] 李青春[1] 杨芳[1] 林少春[2] 吴开力[2] 

机构地区：[1]咸宁学院眼科,湖北咸宁437100 [2]中山大学中山眼科中心,广东广州510060

出　　处：《南方医科大学学报》2012年第1期97-100,共4页Journal of Southern Medical University

基　　金：湖北省卫生厅基金(QJX2008-37;JX2C40);湖北省咸宁学院基金(KY0571)

摘　　要：目的观察汉防己甲素(Tet)对体外培养的人眼Tenon囊成纤维细胞(tenon's capsule fibroblasts,TCFS)中bax、bcl-2、转化生长因子-β2(TGF-β2)mRNA表达的影响,探讨其可能的作用机制。方法将体外培养的第3代TCFS分别置于浓度为10-5mol/LTet的培养液和1640培养液中培养48 h,采用实时荧光定量PCR检测细胞中bax、bcl-2和TGF-β2 mRNA的表达变化。结果同对照组比较,Tet实验组中bax mRNA的表达量明显升高,bcl-2、TGF-β2 mRNA的表达量显著下降,差异均有统计学意义(P<0.05)。结论一定浓度的汉防己甲素可以通过降低bcl-2、TGF-β2 mRNA的表达量,同时提高bax mRNA的表达量来抑制TCFS的增殖,并可诱导其凋亡,可能成为一种潜在的抗青光眼滤过瘢痕药物。Objective To investigate the effect of tetrandrine（Tet） on the expression of bax,bcl-2,and transforming growth factor-β2（TGF-β2） mRNA in cultured human fibroblasts of Tenon＇s capsules（TCFS） and explore its possible mechanism.Methods The third passage of TCFS cultured in vitro were exposed to 1×10-5 mol/L Tet for 24 h,and real-time fluorescence quantitative PCR was used to detect the changes in the expressions of bax,bcl-2,and TGF-β2 mRNA.Results The expression level of bax mRNA was obviously higher,while bcl-2 and TGF-β2 mRNA levels were significantly lower in Tet-treated TCFS than those in the control cells（P0.05）.Conclusion Tet can inhibit the proliferation of TCFS possibly by reducing the expressions of bcl-2 and TGF-β2 mRNA,enhancing the expression of bax mRNA and inducing cell apoptosis,suggesting its potential in preventing fibrous scar formation after glaucoma filtration surgery.

关 键 词：汉防己甲素 TENON囊成纤维细胞 BAX BCL-2 转化生长因子-Β2 实时荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]