泥鳅血清凝集素的研究  

Preliminary study on lectins from serum of loach Misgurnus anguillicaudatus

作　　者：姚东瑞[1,2] 盘赛昆[1] 周鸣谦[1,2] 葛荣荣[1] 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]江苏海洋资源研究院,连云港222005

出　　处：《渔业科学进展》2011年第6期43-49,共7页Progress in Fishery Sciences

基　　金：江苏省科技支撑计划(农业)项目(BE2010399);江苏省农业科技自主创新基金项目[CX09-627;CX(10)328];连云港市科技基础设施建设计划项目(CK0935)共同资助

摘　　要：采用DEAE-SepharoseFF离子交换层析和Superdex20010/300GL凝胶过滤层析技术,从泥鳅血清中分离出两种天然凝集素MSL-I和MSL-Ⅱ。SDS-PAGE检测表明,MSL-I及MSL-Ⅱ均显示两条蛋白质着色带。采用凝胶过滤法测得MSL-I相对分子质量为25000,MSL-Ⅱ相对分子质量为82000。根据凝胶过滤测定及SDS-PAGE检测结果可知,MSL-Ⅱ由相对分子质量分别为29000和13600两种亚基组成,MSL-I的亚基组成有待进一步研究。MSL-Ⅱ能结合多种糖,其中对乳糖的亲和力最强。MSL-Ⅱ具有较强的热稳定性,在pH4～11范围内具有凝集活性,最适pH为6～8。Two lectins with different molecular mass, designated as MSL-I and MSL-II, were isolated from serum of loach Misgurnus anguillicaudatus. The purification procedure con- sisted of separation on DEAE-Sepharose FF anion exchange column followed by gel filtration chromatography on Superdex 200 10/300 GL. Determination showed that MSL-I and MSL-II had a nature molecular weight of 25,000 and 82,000 respectively. The denatured SDS-PAGE showed that both MSL-I and MSL-II had two bands on the gel. We proposed that MSL-II was constituted with two subunits with molecular mass of 29,000 and 13,600 respectively. The sub- unit composition of MSL-I was to be further studied. Sugar-binding specificity test showed that glu- cose, D-galactose, D-fructose, D-xylose, L-arabinose and lactose had inhibitory effect on MSL-II, and among them lactose was most effective. Thermal stability test showed that MSL-II had a strong thermal stability even treated at 60℃ for 30min. The optimum pH of MSL-II was 6-8.

关 键 词：泥鳅 血清 凝集素 分离纯化 

分 类 号：Q965[生物学—昆虫学]