白斑综合征病毒PCR检测中使用dUTP扩增的PCR产物极限长度  

The utmost length of PCR product in detection of white spot syndrome virus(WSSV) using dUTP

作　　者：于洪涛[1,2] 张秀丽[1] 黄倢 张士璀[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]中国海洋大学,青岛266003

出　　处：《渔业科学进展》2011年第6期63-68,共6页Progress in Fishery Sciences

基　　金：国家重点基础研究发展计划(2012CB114401);中央级公益性科研院所基本科研业务费专项资金(2009-ts-01)共同资助

摘　　要：依据白斑综合征病毒的序列设计了7对PCR引物,扩增长度从600bp到1800bp。结果显示,使用dUTP代替dTTP,用普通Taq酶所能扩增的最大长度约为1400bp。Mg2+梯度研究发现,扩增片段长度越长,最适Mg2+浓度越低。使用具有3’-5’外切酶活性的Taq酶无法扩增出任何长度的片段。To prevent carry-over contamination, dTTP was replaced by dUTP in PCR detection for white spot syndrome virus （WSSV）. Seven pairs of primers were designed according to the genome sequence of WSSV. The 600-1 800bp DNA fragments were amplified separately. It was found that the utmost length of PCR product is about 1 400bp, when dUTP is used to take place of dTTP.

关 键 词：dUTP WSSV PCR 极限长度 

分 类 号：S945[农业科学—水产养殖]