剑麻斑马纹病病原鉴定  

Pathogen identification of zebra disease of sisal

作　　者：郑金龙[1,2] 高建明[1] 张世清[1] 陈河龙[1] 刘巧莲[1] 易克贤[1,2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《东北农业大学学报》2011年第12期59-64,共6页Journal of Northeast Agricultural University

基　　金：中央级公益性科研院所基本科研业务费专项(ITBBZx1051);948项目(2010-Z6);国家麻类现代农业产业技术体系建设专项(NYCYTX-19);国家支撑计划项目(2007BAD48B06-05);海南省自然科学基金项目(80510)

摘　　要：剑麻斑马纹病是剑麻的毁灭性真菌病害,是剑麻最严重的病害之一。从海南、广东和广西的剑麻主产区共收集剑麻斑马纹病样品12份,提取其病原菌的基因组DNA并进行rDNA-ITS区序列扩增,序列的比对结果与形态学的鉴定结果相一致,表明参试菌株的病原菌均为烟草疫霉菌(Phytophthora nicotianae Breda);N-J法构建的系统发育树结果表明,12个参试菌株可分为两个类群,存在一定的地域分化。以上研究将为今后对剑麻斑马纹病的发病规律、防治方法及抗病机制等方面的研究奠定基础,提供了理论依据。Zebra spot disease caused by Phytophthora nicotianae is the most serious and devastating fungi disease in sisal. 12 diseased samples were collected from the main sisal production areas in Hainan, Guangdong and Guangxi. The genomic DNA from these samples were extracted, and then rDNA-ITS were amplified. Sequence analysis of the results using morphological identification consistent （comply） with the results, indicated that the strains tested were Phytophthora nicotianae Breda; NJ phylogenetic tree was constructed the result showed that 12 tested isolates could be divided into two groups, and have some genetic differences. Future research would provie a theoretical basis for future research in sisal law zebra spot disease occurrence regularity, prevention methods and disease resistance mechanisms basis.

关 键 词：剑麻 斑马纹病 rDNA—ITS 序列分析 病原鉴定 

分 类 号：S563.8[农业科学—作物学] S435.63