生长相关蛋白在电针治疗大鼠脊髓损伤中的表达及意义  

Expression of Growth Associated Protein in Rats of Spinal Cord Injury Treated with Electro-acupuncture and Its Significance

作　　者：李振鹏[1] 孔抗美[1] 邹延澄[1] 郭天明[1] 林耿春[1] 

机构地区：[1]汕头大学医学院第二附属医院骨科,广东汕头515041

出　　处：《医学临床研究》2011年第12期2237-2239,共3页Journal of Clinical Research

基　　金：广东省卫生厅资助课题（项目编号：A2009445）

摘　　要：[目的]探讨生长相关蛋白（Gap-43）在电针治疗大鼠脊髓损伤（SCI）中的变化及意义.[方法]以2个月龄Wistar大鼠（96只）为受试对象,随机分为对照组（A组）、损伤组（B组）、治疗对照组（C组）和电流刺激治疗组（D组）.B、C、D组于T9处行脊髓全横断,D组予电针治疗.用Western-blot测定各组损伤段脊髓中Gap-43不同时相点表达量及其变化,以苏木精-伊红和免疫组化染色对受损脊髓进行形态学观察.[结果]SCI后Gap-43有少量表达,经过电针治疗后,表达显著增多.[结论]Gap-43是SCI后神经纤维再生的重要因子,电针治疗对其表达具有明显的促进作用.[Objective] To explore the change of growth associated protein（Gal〉43） in rats of spinal cord injury （SCI） treated with electro-acupuncture and its significance. [Methods] Totally 96 Wistar rats with 2-month age were selected as the subjects and randomly divided into the control group（group A）, SCI group（group B）, the sham-treatment group（group C） and the electro-acupuncture treatment group（group D）. Group B, C and D underwent total-transect SCI at the vertebrae level of T9. Group D was treated with electro-acupuncture. Western-blot was used to examine the expression and change of Gap-3 in injured spinal cord at different time points. Hematoxylin-feosin and immunohistochemical staining methods were used to observe the morphology of injured spinal cord. [Results]A little expression of Gap-43 after SCI was found. After electro-acupuncture treatment, the expression of Gap-43 increased obviously. [Conclusion] Gap-43 is an important factor of nerve fiber regeneration after SCI. Electro-acupuncture can obviously promote the expression of Gap- 43 protein.

关 键 词：脊髓损伤/治疗 神经组织蛋白质类 电针 大鼠 

分 类 号：R651.2[医药卫生—外科学]