IL-8对血管内皮细胞紧密连接的影响  

Effects of IL-8 on the tight junction of vascular endothelial cells

作　　者：俞燚[1] 刘肖珩[1] 沈阳[1] 高亭[1] 高敏[1] 马云龙[1] 付强[2] 康敏[2] 赵健民[2] 

机构地区：[1]四川大学华西基础医学与法医学院生物工程研究室,成都610041 [2]西藏大学医学院,拉萨850002

出　　处：《国际生物医学工程杂志》2011年第6期336-339,I0009,共5页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（10772127,10972148）;四川省青年基金资助项目（06ZQ026-009）;教育部新世纪优秀人才支持计划资助项目（NCET-06-0789）

摘　　要：目的研究白细胞介素-8（IL8）对血管内皮细胞紧密连接的影响。方法采用免疫荧光染色检测经不同浓度和时间IL-8处理后EA．hy926细胞的3种紧密连接蛋白occludin、claudin-5和ZO一1的形态和分布；逆转录PCR（RT-PCR）检测3种蛋白mRNA的表达水平。结果IL-8可改变内皮细胞紧密连接蛋白occludin、claudin．5和ZO-1的分布和表达强度；随着IL-8浓度和作用时间的增加，occludin、claudin-5和ZO-1mRNA表达逐渐降低。结论IL-8改变occludin、claudin-5和ZO-13种紧密连接蛋白的分布和表达，并下调其mRNA表达，且呈现出一定的时间和浓度依赖关系。cells. Methods Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial was used to observe the modality and the distribution of three tight junction proteins （occludin,claudin-5 and ZO-1） of the EA.hy926 cells treated with IL-8 under different concentrations and different times. RT-PCR was used to measure the mRNA expression of these three proteins. Results The results demonstrated that IL-8 could change the distribution of occludin, claudin-5 and ZO-1 in EA.hy926 cells, and the mRNA expression of occludin, claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time. Conclusion The effects of IL-8 on the distribution and the expression of occludin, claudin-5 and ZO-1 are dose and time-dependent.

关 键 词：白细胞介素-8 血管内皮细胞 紧密连接 

分 类 号：R285.5[医药卫生—中药学]