大豆对SMV1号株系的抗性遗传分析及抗病基因的SSR标记  

Genetic analysis of resistance and identification of SSR marker linked to the resistance gene of soybean(Glycine max L.Merr.) to SMV1

作　　者：滕卫丽[1] 韩英鹏[1] 李文滨[1] 

机构地区：[1]东北农业大学大豆研究所,大豆生物学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《中国油料作物学报》2011年第6期555-560,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家863计划(2006AA10Z1F1);东北农业大学科学研究基金;黑龙江省教育厅科学技术研究(11531020)

摘　　要：利用杂交组合合丰25(S)×东农93-046(R)的F1、F2和F2∶3群体进行了抗病性鉴定,结果表明:F1在接种后表现抗病,F2群体分离比例为3(抗)∶1(感),对F2衍生的F2∶3家系接种鉴定,纯合抗病家系、抗感分离家系和纯合感病家系的比率符合1∶2∶1,表明东农93-046对SMV1号株系的抗性由一对显性基因(RSMV1)控制。并利用东农93-046(R)×Conrad(S)的正反交组合F2进行抗性鉴定,结果正反交后代均表现为3∶1的分离比例,无细胞质效应,进一步证明了东农93-046是受一对基因控制的抗性种质。经改良的分离群体组群分析法研究发现,东农93-046抗SMV1的位点(RSMV1)位于F连锁群上,与SSR标记的连锁顺序为HSP176、Satt114、RSMV1、Satt510、Sct_033、Satt334、Satt362,标记与RSMV1之间的遗传距离分别为10.9cM、4.3cM、6.6cM、11.7cM、18.0cM和35.3cM。根据标记HSP176选择基因型纯合和杂合的抗病植株,其符合率分别达到100.0%和94.5%。Soybean resistance to SMV1 was investigated by artificial inoculation.F1,F2 and F2∶ 3 populations from Hefeng25（S） × Dongnong93-046（R） were used as materials.Result showed that F1 had the resistance.Segregation ratio of 225 F2 populations was 3（resistant and necrosis）∶ 1（susceptible）.The ratio of F2∶ 3 families were 1（resistant）∶ 2（heterozygous）∶ 1（susceptible）.Results also showed that Dongnong93-046 had a single dominant resistant gene to SMV1.Investigation of reciprocal crosses between Dongnong93-046（R） and Conrad（S） proved that resistance to susceptibility ratio was 1∶ 1.No cytoplasmic effect was found which indicated that Dongnong93-046 was a highly resistant accession,and a single dominant gene conferred plant resistance to SMV1.Modified bulked segregation analysis（BSA） demonstrated that 6 SSR markers in F linkage group were closely linked to RSMV1 in F2 mapping population from Hefeng25 × Dongnong93-046.The orientation of RSMV1 and 6 SSR markers was determined as HSP176（10.9cM）,Satt114（4.3cM）,RSMV1（0.0cM）,Satt510（6.6cM）,Sct_033（11.7cM）,Satt334（18.0cM）,Satt362（35.3cM）.Coincidence rates of selecting homozygous and heterozygous resistant plants were 100% and 94.5% respectively using HSP176 marker.

关 键 词：大豆 SMV1 遗传分析 SSR 分子标记 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]