内标校准液相色谱荧光法测定食用油中苯并(a)芘  

Determination of benzo[a]pyrene in edible oils by liquid chromatographyfluorescence detection with benzo[b]chrysene as internal standard

作　　者：王秀嫔[1,2,3] 李培武[1,2,3,4] 王琳[1,3] 张文[1,3,4] 张奇[1,3] 甘冬生[1,3] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点开放实验室,湖北武汉430062 [3]农业部生物毒素检测重点实验室,湖北武汉430062 [4]农业部油料及制品质量监督检验测试中心,湖北武汉430062

出　　处：《中国油料作物学报》2011年第6期603-608,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家油菜现代产业技术体系(cars-13);国家自然科学基金(31000877);农业部948项目(2010-G1;2011-G5);国家科技支撑计划(2010BAK08B03;2012BAB19B09);中国农业科学院油料作物研究所所长基金

摘　　要：采用内标苯并(b)屈校正,建立了正相固相净化-反相液相色谱-荧光分光光度法测定食用油中苯并(a)芘的方法。正相Plus Silica固相萃取柱作为净化柱,反相C18柱作为分离柱。以苯并(b)屈作为内标,测定了苯并(a)芘的校正因子。使用本方法,苯并(a)芘在液态食用油和固态食用油中的检出限分别为0.01和0.03μg/kg。苯并(a)芘在不同食用油回收率在83%～108%之间,方法的日内精密度和日间精密度分别小于7.7%和13.2%。The normal-phase solid phase cleaning-reversed high performance liquid chromatography-fluorescence detection was applied for the determination of the benzo[a]pyrene（BaP） in edible oils.The edible oil samples were cleansed with a Plus Silica solid phase extraction cartridge.The separation was carried out on a C18 column with gradient elution.With benzo[b]chrysene（BbC） as the internal standard,the relative correction factor（RCF） of BaP was determined by HPLC-FLD;the BaP was determined by using BbC as the single marker.The limits of detection were 0.01μg/kg and 0.03μg/kg for BaP in edible oils and in edible fats,respectively.Recoveries of BaP in different spiked edible oils were in the range from 83% to 108%.The intra-day precision and inter-day precision were less than 7.7% and 13.2% respectively.

关 键 词：苯并(A)芘 苯并(b)屈 内标 正相固相净化 HPLC-FLD 

分 类 号：S132[农业科学—农业基础科学]