脂联素水平及其基因+45T/G和+276G/T与原发性高血压的相关性  

Correlation of the Adiponectin Level and Gene Polymorphism with the Essential Hypertension

作　　者：陈芳[1] 朱红[1] 武海亮[2] 杨锐英[2] 

机构地区：[1]银川市第一人民医院,银川750001 [2]宁夏医科大学总医院,银川750004

出　　处：《宁夏医科大学学报》2011年第11期1019-1022,共4页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金项目(NZ09109)

摘　　要：目的探讨脂联素(APN)水平及其单核苷酸基因多态性(SNP)+45T/G和+276G/T两个位点与原发性高血压(EH)的相关性。方法 238例研究对象分为对照组、原发性高血压组,采用实验-对照研究方法,应用酶联免疫吸附法(ELISA)及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对血浆脂联素浓度及脂联素两个位点基因多态性进行检测。结果与对照组相比EH组APN降低(P<0.05);EH组SNP+276T等位基因频率明显升高,多元线性回归分析显示+276基因多态性为EH危险因素。结论 EH患者APN水平下降,携带+276T等位基因者患EH的风险增加。Objective To explore the relationship of the adiponectin（APN） level,single nucleotide polymorphism（SNP） ＋45 T / G and ＋276 G/T with the essential hypertension（EH）.Methods 238 subjects were divided into control group and EH group.Experimental-control study was used.Concentration of the plasma adiponection and polymorphisms of the two polymorphisms of adiponectin were detected with enzyme-linked immunosorbent assay（ELISA） and polymerase chain reaction-restriction fragment length polymorphism（PCR-RELP）.Results Comparing with the control group,APN of the EH group significantly reduced and the genotype frequency of EH group on SNP＋276T alleles rised significantly,Multiple linear regression（MLR） analysis showed that SNPS ＋276 locus was risk factor of EH.Conclusion APN level of patients with EH decline;People who carries the SNP ＋ 276T alleles run more risk at EH.

关 键 词：脂联素 原发性高血压 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]